Interaction of Sec4 with GDI proteins from bovine brain, Drosophila melanogaster and Saccharomyces cerevisiae. Conservation of GDI membrane dissociation activity

Michelle D. Garrett, Alisa K. Kabcenell, Joseph E. Zahner, Kozo Kaibuchi, Takuya Sasaki, Yoshimi Takai, Clarissa M. Cheney, Peter J. Novick

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Rab GDP dissociation inhibitor (Rab GDI), will induce the dissociation of GDP-bound rab3A from synaptic membranes and will inhibit GDP dissociation from Sec4, a member of the Rab subgroup of the Ras GTPase superfamily which is required for exocytosis in Saccharomyces cerevisiae. We report that Rab GDI releases GDP-bound Sec4 from yeast membranes. dGDI, a Drosophila homologue can similarly inhibit GDP dissociation from Sec4 and release GDP-bound Sec4 from yeast membranes. An activity partially purified from yeast cytosol dissociates GDP-bound Sec4 from yeast membranes, suggesting that yeast also possess a GDI protein that functions to recycle Sec4 from its target membrane.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalFEBS Letters
Volume331
Issue number3
DOIs
Publication statusPublished - 04-10-1993

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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