Interaction of von Willebrand factor with the extracellular matrix and glycocalicin under static conditions

Taei Matsui, Shinji Kunishima, Jiharu Hamako, Masahiko Katayama, Tadashi Kamiya, Tomoki Naoe, Yasuhiro Ozeki, Yoshihiro Fujimura, Koiti Titani

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The binding of human von Willebrand factor (vWF) to a variety of extracellular matrix components immobilized on plates and the binding of vWF to platelet glycoprotein Ib (GPIb) after interacting with these matrix components were examined by means of an enzyme-linked immunosorbent assay. vWF preferably bound to type III collagen, whereas it did not significantly bind to type I, IV, V, or VI collagen, fibronectin, laminin, elastin, or proteoglycans. Soluble type III collagen did not bind to vWF coated on plates and showed a little effect on the vWF binding to the immobilized collagen, suggesting that solid-phase collagen is important for the interaction with vWF. When glycocalicin, the N-terminal carbohydrate-rich extracellular domain of GPIbα exhibiting the vWF-binding activity, was added to vWF bound to collagen type III, no significant binding of glycocalicin was observed, but it bound to vWF in the presence of botrocetin, a vWF modulator protein isolated from Bothrops jararaca snake venom. These results indicate that vWF immobilized on collagen can interact with GPIb but that the binding of vWF to the collagen matrix alone is insufficient for modulating vWF so that it interacts with GPIb under static conditions. Another unknown physiological modulator functionally mimicking botrocetin or high-shear stress may be involved in the platelet adhesion to extracellular matrix in the early stage of hemostasis.

Original languageEnglish
Pages (from-to)376-381
Number of pages6
JournalJournal of Biochemistry
Volume121
Issue number2
DOIs
Publication statusPublished - 01-01-1997

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Interaction of von Willebrand factor with the extracellular matrix and glycocalicin under static conditions'. Together they form a unique fingerprint.

Cite this