TY - JOUR
T1 - Interleukin-1α Enhances Angiogenesis and Is Associated With Liver Metastatic Potential in Human Gastric Cancer Cell Lines
AU - Ma, Jiachi
AU - Sawai, Hirozumi
AU - Matsuo, Yoichi
AU - Ochi, Nobuo
AU - Yasuda, Akira
AU - Takahashi, Hiroki
AU - Wakasugi, Takehiro
AU - Funahashi, Hitoshi
AU - Sato, Mikinori
AU - Okada, Yuji
AU - Takeyama, Hiromitsu
AU - Manabe, Tadao
PY - 2008/8
Y1 - 2008/8
N2 - Background: To better understand the underlying mechanism of liver metastasis formation in human gastric cancer, we evaluated the angiogenic capabilities of human gastric cancer cell lines with different metastatic potentials as well as the role of interleukin (IL)-1α in the angiogenic process. Materials and methods: Reverse transcription-polymerase chain reaction was used to detect the expression of IL-1α and vascular endothelial growth factor (VEGF) mRNA in gastric cancer cell lines with different liver metastatic potentials. Levels of VEGF secreted by human gastric cancer cells were measured by enzyme-linked immunosorbent assay. We also examined how gastric cancer cells with different metastatic potentials influence the proliferation and tube formation of human umbilical vein endothelial cells (HUVECs) using the Premix WST-1 cell proliferation assay system and an angiogenesis assay, respectively. Results: IL-1α expression levels were significantly correlated with liver metastatic potential in gastric cancer cell lines. Levels of VEGF secreted by gastric cancer cells appear to be regulated by IL-1α through IL-1 receptor Type 1 and were correlated with liver metastatic potential. Both HUVEC proliferation and tube formation were strongly enhanced by coculture with high liver-metastatic gastric cancer cells and were enhanced to a similar extent by culture in the presence of IL-1α. In contrast, blockade of IL-1α inhibited both HUVEC proliferation and angiogenesis. Conclusions: IL-1α may play a role in liver metastasis of gastric cancer via enhanced vascular endothelial cell proliferation and angiogenesis.
AB - Background: To better understand the underlying mechanism of liver metastasis formation in human gastric cancer, we evaluated the angiogenic capabilities of human gastric cancer cell lines with different metastatic potentials as well as the role of interleukin (IL)-1α in the angiogenic process. Materials and methods: Reverse transcription-polymerase chain reaction was used to detect the expression of IL-1α and vascular endothelial growth factor (VEGF) mRNA in gastric cancer cell lines with different liver metastatic potentials. Levels of VEGF secreted by human gastric cancer cells were measured by enzyme-linked immunosorbent assay. We also examined how gastric cancer cells with different metastatic potentials influence the proliferation and tube formation of human umbilical vein endothelial cells (HUVECs) using the Premix WST-1 cell proliferation assay system and an angiogenesis assay, respectively. Results: IL-1α expression levels were significantly correlated with liver metastatic potential in gastric cancer cell lines. Levels of VEGF secreted by gastric cancer cells appear to be regulated by IL-1α through IL-1 receptor Type 1 and were correlated with liver metastatic potential. Both HUVEC proliferation and tube formation were strongly enhanced by coculture with high liver-metastatic gastric cancer cells and were enhanced to a similar extent by culture in the presence of IL-1α. In contrast, blockade of IL-1α inhibited both HUVEC proliferation and angiogenesis. Conclusions: IL-1α may play a role in liver metastasis of gastric cancer via enhanced vascular endothelial cell proliferation and angiogenesis.
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U2 - 10.1016/j.jss.2007.08.014
DO - 10.1016/j.jss.2007.08.014
M3 - Article
C2 - 18395750
AN - SCOPUS:46249097170
SN - 0022-4804
VL - 148
SP - 197
EP - 204
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 2
ER -
