Intracellular interferon-γ staining analysis of donor-specific t-cell responses in liver transplant recipients

Y. Okanami, K. Tsujimura, S. Mizuno, M. Tabata, S. Isaji, Y. Akatsuka, K. Kuzushima, T. Takahashi, S. Uemoto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Objective: Biomarkers that accurately reflect, detect, and/or predict detrimental immune responses to grafts are important in organ transplantation. We established a new detection method for alloreactive T cells on the basis of intracellular staining for interferon (IFN)-γ, using CD40-activated B cells as stimulators, and assessed temporal changes in alloreactive T-cell frequencies in patients who received liver transplantation. Methods: Peripheral blood mononuclear cells and CD40-activated B cells were used as responder and stimulator cells, respectively. The responder cells were cultured with the stimulator cells for 7 days, restimulated for 5 hours, and flow cytometrically tested by intracellular staining for IFN-γ. Results: The relative postoperative-preoperative ratio of donor-specific CD8 + T cells in the nonrejection group was significantly lower than that in the rejection group and found to be <1 in most individuals of the group throughout the postoperative periods, indicating an induction of donor-specific suppression of the CD8 + T-cell responses. In contrast, such differences were not found in the donor-specific CD4 + T cells. These results suggest that the relative postoperativepreoperative ratio of the donor-specific CD8 + T cells is a good indicator of graft rejection. Conclusion: We established a new flow cytometric method for the detection of alloreactive T cells by intracellular staining for IFN-γ, using CD40-activated B cells as stimulator cells. Using this system, we found that the relative postoperativepreoperative ratio of the donor-specific CD8 + T cells is a possible evaluative indicator of the risk for graft rejection.

Original languageEnglish
Pages (from-to)548-554
Number of pages7
JournalTransplantation Proceedings
Volume44
Issue number2
DOIs
Publication statusPublished - 03-2012

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

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