Intratumoral evaluation of 3D microvasculature and nanoparticle distribution using a gadolinium-dendron modified nano-liposomal contrast agent with magnetic resonance micro-imaging

Nobuhiro Nitta, Yoichi Takakusagi, Daisuke Kokuryo, Sayaka Shibata, Akihiro Tomita, Tatsuya Higashi, Ichio Aoki, Masafumi Harada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The enhanced permeability and retention (EPR) effect is variable depending on nanoparticle properties and tumor/vessel conditions. Thus, intratumoral evaluations of the vasculature and nanoparticle distribution are important for predicting the therapeutic efficacy and the intractability of tumors. We aimed to develop a tumor vasculature evaluation method and high-resolution nanoparticle delivery imaging using magnetic resonance (MR) micro-imaging technology with a gadolinium (Gd)-dendron assembled liposomal contrast agent. Using the Gd-liposome and a cryogenic receiving coil, we achieved 50-μm isotropic MR angiography with clear visualization of tumor micro-vessel structure. The Gd-liposome-enhanced MR micro-imaging revealed differences in the vascular structures between Colon26- and SU-DHL6-grafted mice models. The vessel volumes and diameters measured for both tumors were significantly correlated with histological observations. The MR micro-imaging methods facilitate the evaluation of intratumoral vascularization patterns, the quantitative assessment of vascular-properties that alter tumor malignancy, particle retentivity, and the effects of treatment.

Original languageEnglish
Pages (from-to)1315-1324
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume14
Issue number4
DOIs
Publication statusPublished - 01-06-2018

Fingerprint

Gadolinium
Magnetic resonance
Microvessels
Nanoparticles
Contrast Media
Tumors
Magnetic Resonance Imaging
Imaging techniques
Neoplasms
Liposomes
Blood Vessels
Angiography
Magnetic Resonance Angiography
Cryogenics
dendron
Visualization
Permeability
Technology
Therapeutics

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Nitta, Nobuhiro ; Takakusagi, Yoichi ; Kokuryo, Daisuke ; Shibata, Sayaka ; Tomita, Akihiro ; Higashi, Tatsuya ; Aoki, Ichio ; Harada, Masafumi. / Intratumoral evaluation of 3D microvasculature and nanoparticle distribution using a gadolinium-dendron modified nano-liposomal contrast agent with magnetic resonance micro-imaging. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2018 ; Vol. 14, No. 4. pp. 1315-1324.
@article{b029884fe4134c4696f8fbe1dadce4d6,
title = "Intratumoral evaluation of 3D microvasculature and nanoparticle distribution using a gadolinium-dendron modified nano-liposomal contrast agent with magnetic resonance micro-imaging",
abstract = "The enhanced permeability and retention (EPR) effect is variable depending on nanoparticle properties and tumor/vessel conditions. Thus, intratumoral evaluations of the vasculature and nanoparticle distribution are important for predicting the therapeutic efficacy and the intractability of tumors. We aimed to develop a tumor vasculature evaluation method and high-resolution nanoparticle delivery imaging using magnetic resonance (MR) micro-imaging technology with a gadolinium (Gd)-dendron assembled liposomal contrast agent. Using the Gd-liposome and a cryogenic receiving coil, we achieved 50-μm isotropic MR angiography with clear visualization of tumor micro-vessel structure. The Gd-liposome-enhanced MR micro-imaging revealed differences in the vascular structures between Colon26- and SU-DHL6-grafted mice models. The vessel volumes and diameters measured for both tumors were significantly correlated with histological observations. The MR micro-imaging methods facilitate the evaluation of intratumoral vascularization patterns, the quantitative assessment of vascular-properties that alter tumor malignancy, particle retentivity, and the effects of treatment.",
author = "Nobuhiro Nitta and Yoichi Takakusagi and Daisuke Kokuryo and Sayaka Shibata and Akihiro Tomita and Tatsuya Higashi and Ichio Aoki and Masafumi Harada",
year = "2018",
month = "6",
day = "1",
doi = "10.1016/j.nano.2018.03.006",
language = "English",
volume = "14",
pages = "1315--1324",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",
number = "4",

}

Intratumoral evaluation of 3D microvasculature and nanoparticle distribution using a gadolinium-dendron modified nano-liposomal contrast agent with magnetic resonance micro-imaging. / Nitta, Nobuhiro; Takakusagi, Yoichi; Kokuryo, Daisuke; Shibata, Sayaka; Tomita, Akihiro; Higashi, Tatsuya; Aoki, Ichio; Harada, Masafumi.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 14, No. 4, 01.06.2018, p. 1315-1324.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Intratumoral evaluation of 3D microvasculature and nanoparticle distribution using a gadolinium-dendron modified nano-liposomal contrast agent with magnetic resonance micro-imaging

AU - Nitta, Nobuhiro

AU - Takakusagi, Yoichi

AU - Kokuryo, Daisuke

AU - Shibata, Sayaka

AU - Tomita, Akihiro

AU - Higashi, Tatsuya

AU - Aoki, Ichio

AU - Harada, Masafumi

PY - 2018/6/1

Y1 - 2018/6/1

N2 - The enhanced permeability and retention (EPR) effect is variable depending on nanoparticle properties and tumor/vessel conditions. Thus, intratumoral evaluations of the vasculature and nanoparticle distribution are important for predicting the therapeutic efficacy and the intractability of tumors. We aimed to develop a tumor vasculature evaluation method and high-resolution nanoparticle delivery imaging using magnetic resonance (MR) micro-imaging technology with a gadolinium (Gd)-dendron assembled liposomal contrast agent. Using the Gd-liposome and a cryogenic receiving coil, we achieved 50-μm isotropic MR angiography with clear visualization of tumor micro-vessel structure. The Gd-liposome-enhanced MR micro-imaging revealed differences in the vascular structures between Colon26- and SU-DHL6-grafted mice models. The vessel volumes and diameters measured for both tumors were significantly correlated with histological observations. The MR micro-imaging methods facilitate the evaluation of intratumoral vascularization patterns, the quantitative assessment of vascular-properties that alter tumor malignancy, particle retentivity, and the effects of treatment.

AB - The enhanced permeability and retention (EPR) effect is variable depending on nanoparticle properties and tumor/vessel conditions. Thus, intratumoral evaluations of the vasculature and nanoparticle distribution are important for predicting the therapeutic efficacy and the intractability of tumors. We aimed to develop a tumor vasculature evaluation method and high-resolution nanoparticle delivery imaging using magnetic resonance (MR) micro-imaging technology with a gadolinium (Gd)-dendron assembled liposomal contrast agent. Using the Gd-liposome and a cryogenic receiving coil, we achieved 50-μm isotropic MR angiography with clear visualization of tumor micro-vessel structure. The Gd-liposome-enhanced MR micro-imaging revealed differences in the vascular structures between Colon26- and SU-DHL6-grafted mice models. The vessel volumes and diameters measured for both tumors were significantly correlated with histological observations. The MR micro-imaging methods facilitate the evaluation of intratumoral vascularization patterns, the quantitative assessment of vascular-properties that alter tumor malignancy, particle retentivity, and the effects of treatment.

UR - http://www.scopus.com/inward/record.url?scp=85046444189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046444189&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2018.03.006

DO - 10.1016/j.nano.2018.03.006

M3 - Article

C2 - 29626524

AN - SCOPUS:85046444189

VL - 14

SP - 1315

EP - 1324

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

IS - 4

ER -