Introduction of the c-kit gene leads to growth suppression of a breast cancer cell line, MCF-7

Keiko Nishida, Tetsuya Tsukamoto, Kosaku Uchida, Toshitada Takahash, Takashi Takahashi, Ryuzo Ueda

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18 Citations (Scopus)

Abstract

Normal ductal cells of the breast are exceptional in that their epithelial cells abundantly express the c-kit receptor. Loss of c-kit expression has been reported in 80-90% of breast cancer specimens, suggesting a possible role in the development of tumors. In the present study, we introduced a c-kit expression vector vector into breast cancer cell line. MCF-7, which does not express c-kit does express its ligand, stem cell factor (SCF). Anchorage dependent and independent growth was found to be inhibited in bulk cultures of the c-kit transfectants, although this suppression appeared to be incomplete, allowing a considerably fraction to tolerate c-kit expression. Heterogenous sensitivity to the suppressive effects mediated by the c-kit receptor was also observed among individual clones isolated from the bulk cultures. These results suggest that c-kit can mediated inhibitory signals for the growth of breast cancer cells, but that cellular heterogeneity exists regarding the response. Further studies are warranted to elucidate the molecular basis for the inhibitory effects of c-kit.

Original languageEnglish
Pages (from-to)3397-3402
Number of pages6
JournalAnticancer research
Volume16
Issue number6 B
Publication statusPublished - 01-12-1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Nishida, K., Tsukamoto, T., Uchida, K., Takahash, T., Takahashi, T., & Ueda, R. (1996). Introduction of the c-kit gene leads to growth suppression of a breast cancer cell line, MCF-7. Anticancer research, 16(6 B), 3397-3402.