TY - JOUR
T1 - Involvement of GANP in B cell activation in T cell-dependent antigen response
AU - Sakaguchi, Nobuo
AU - Fujimura, Satoru
AU - Kuwahara, Kazuhiko
N1 - Funding Information:
Sakaguchi Nobuo 1, 2 Fujimura Satoru 1 Kuwahara Kazuhiko 1, 3 1 Department of Immunology Graduate School of Medical Sciences Kumamoto University 1-1-1 Honjo, Kumamoto 860-0811 Japan kumamoto-u.ac.jp 2 CREST Japan Science and Technology Corporation (JST) Japan jst.go.jp 3 PRESTO Japan Science and Technology Corporation (JST) Japan jst.go.jp 2002 9 3 169 172 2002 Copyright © 2002 Hindawi Publishing Corporation Adaptive immunity is dependent on proliferation of antigen-driven B cells for clonal expansion in germinal centers (GCs) against T cell-dependent antigens (TD-Ag), accompanied with somatic hypermutation of variable-region gene and class switching of B cell antigen receptors. To study molecular mechanisms for B cell differentiation in GCs, we have identified and studied a 210 kDa GANP protein expressed in GC-B cells. GANP has domains for MCM3-binding and RNA-primase activities and is selectively up-regulated in centrocytes surrounded with follicular dendritic cells (FDCs) upon immunization with TD-Ag in vivo and in B cells stimulated with anti-CD40 monoclonal antibody in vitro , which suggested that GANP plays a certain important role in the maturation of immunoglobulin or selection of B cells in GC during the immune response to TD-Ag. Since this up-regulation has not been detected in T cells in GCs and in Concanavalin A-stimulated T cells in vitro , selective function of GANP molecule on B cell proliferation and differentiation might exist. http://dx.doi.org/10.13039/501100001700 Ministry of Education, Culture, Sports, Science, and Technology Uehara Memorial Foundation
PY - 2002/9
Y1 - 2002/9
N2 - Adaptive immunity is dependent on proliferation of antigen-driven B cells for clonal expansion in germinal centers (GCs) against T cell-dependent antigens (TD-Ag), accompanied with somatic hypermutation of variable-region gene and class switching of B cell antigen receptors. To study molecular mechanisms for B cell differentiation in GCs, we have identified and studied a 210kDa GANP protein expressed in GC-B cells. GANP has domains for MCM3-binding and RNA-primase activities and is selectively up-regulated in centrocytes surrounded with follicular dendritic cells (FDCs) upon immunization with TD-Ag in vivo and in B cells stimulated with anti-CD40 monoclonal antibody in vitro, which suggested that GANP plays a certain important role in the maturation of immunoglobulin or selection of B cells in GC during the immune response to TD-Ag. Since this up-regulation has not been detected in T cells in GCs and in Concanavalin A-stimulated T cells in vitro, selective function of GANP molecule on B cell proliferation and differentiation might exist.
AB - Adaptive immunity is dependent on proliferation of antigen-driven B cells for clonal expansion in germinal centers (GCs) against T cell-dependent antigens (TD-Ag), accompanied with somatic hypermutation of variable-region gene and class switching of B cell antigen receptors. To study molecular mechanisms for B cell differentiation in GCs, we have identified and studied a 210kDa GANP protein expressed in GC-B cells. GANP has domains for MCM3-binding and RNA-primase activities and is selectively up-regulated in centrocytes surrounded with follicular dendritic cells (FDCs) upon immunization with TD-Ag in vivo and in B cells stimulated with anti-CD40 monoclonal antibody in vitro, which suggested that GANP plays a certain important role in the maturation of immunoglobulin or selection of B cells in GC during the immune response to TD-Ag. Since this up-regulation has not been detected in T cells in GCs and in Concanavalin A-stimulated T cells in vitro, selective function of GANP molecule on B cell proliferation and differentiation might exist.
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U2 - 10.1080/1044667031000137647
DO - 10.1080/1044667031000137647
M3 - Article
C2 - 12885157
AN - SCOPUS:0042815044
VL - 9
SP - 169
EP - 172
JO - Journal of Immunology Research
JF - Journal of Immunology Research
SN - 2314-8861
IS - 3
ER -