Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells

Etsuko Hirose; Miyoko Matsushima; Kenzo Takagi; Yui Ota; Keiko Ishigami; Tatsuya Hirayama; Yuta Hayashi; Toshinobu Nakamura; Naozumi Hashimoto; Kazuyoshi Imaizumi; Kenji Baba; Yoshinori Hasegawa; Tsutomu Kawabe

doi:10.1007/s10753-009-9108-4

Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells

Etsuko Hirose, Miyoko Matsushima, Kenzo Takagi, Yui Ota, Keiko Ishigami, Tatsuya Hirayama, Yuta Hayashi, Toshinobu Nakamura, Naozumi Hashimoto, Kazuyoshi Imaizumi, Kenji Baba, Yoshinori Hasegawa, Tsutomu Kawabe

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.

Original language	English
Pages (from-to)	99-108
Number of pages	10
Journal	Inflammation
Volume	32
Issue number	2
DOIs	https://doi.org/10.1007/s10753-009-9108-4
Publication status	Published - 04-2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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@article{6317e815faee49f3ab9bf44391f923f0,

title = "Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells",

abstract = "Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.",

author = "Etsuko Hirose and Miyoko Matsushima and Kenzo Takagi and Yui Ota and Keiko Ishigami and Tatsuya Hirayama and Yuta Hayashi and Toshinobu Nakamura and Naozumi Hashimoto and Kazuyoshi Imaizumi and Kenji Baba and Yoshinori Hasegawa and Tsutomu Kawabe",

note = "Funding Information: This work was supported in part by the Grant-in-Aid for the Scientific Research from the Japan Society for the Promotion of Science, and Grant-in-Aid of Nagono Medical Foundation (Nagoya, Japan), and Ichihara International Scholarship Foundation (Nagoya, Japan).",

year = "2009",

month = apr,

doi = "10.1007/s10753-009-9108-4",

language = "English",

volume = "32",

pages = "99--108",

journal = "Inflammation",

issn = "0360-3997",

publisher = "Springer New York",

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TY - JOUR

T1 - Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells

AU - Hirose, Etsuko

AU - Matsushima, Miyoko

AU - Takagi, Kenzo

AU - Ota, Yui

AU - Ishigami, Keiko

AU - Hirayama, Tatsuya

AU - Hayashi, Yuta

AU - Nakamura, Toshinobu

AU - Hashimoto, Naozumi

AU - Imaizumi, Kazuyoshi

AU - Baba, Kenji

AU - Hasegawa, Yoshinori

AU - Kawabe, Tsutomu

N1 - Funding Information: This work was supported in part by the Grant-in-Aid for the Scientific Research from the Japan Society for the Promotion of Science, and Grant-in-Aid of Nagono Medical Foundation (Nagoya, Japan), and Ichihara International Scholarship Foundation (Nagoya, Japan).

PY - 2009/4

Y1 - 2009/4

N2 - Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.

AB - Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.

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JF - Inflammation

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Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells

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