TY - JOUR
T1 - Involvement of MAPK signaling molecules and Runx2 in the NELL1-induced osteoblastic differentiation
AU - Bokui, Nobuyuki
AU - Otani, Takayuki
AU - Igarashi, Koichi
AU - Kaku, Junichiro
AU - Oda, Mitsuo
AU - Nagaoka, Tadahiro
AU - Seno, Masaharu
AU - Tatematsu, Kenji
AU - Okajima, Toshihide
AU - Matsuzaki, Takashi
AU - Ting, Kang
AU - Tanizawa, Katsuyuki
AU - Kuroda, Shun'ichi
N1 - Funding Information:
We thank Prof. Ryutaro Kamijo (Showa University Dental School) for helpful discussion and Prof. Takenobu Katagiri (Saitama Medical University) for supplying the Id1 vectors. This study was supported in part by a Grant-in-Aid from JSPS (to SK; No. 15300126).
PY - 2008/1/23
Y1 - 2008/1/23
N2 - NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rapid intracellular accumulation of Tyr-phosphorylated proteins. Unlike BMP2, NELL1 protein does not activate the Smad signaling cascade. These findings suggest that upon binding to a specific receptor NELL1 transduces an osteogenic signal through activation of certain Tyr-kinases associated with the Ras-MAPK cascade, and finally leads to the osteogenic differentiation.
AB - NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rapid intracellular accumulation of Tyr-phosphorylated proteins. Unlike BMP2, NELL1 protein does not activate the Smad signaling cascade. These findings suggest that upon binding to a specific receptor NELL1 transduces an osteogenic signal through activation of certain Tyr-kinases associated with the Ras-MAPK cascade, and finally leads to the osteogenic differentiation.
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U2 - 10.1016/j.febslet.2007.12.006
DO - 10.1016/j.febslet.2007.12.006
M3 - Article
C2 - 18082140
AN - SCOPUS:38049046208
SN - 0014-5793
VL - 582
SP - 365
EP - 371
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -