TY - JOUR
T1 - Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation
AU - Iwatani, Sota
AU - Shono, Akemi
AU - Yoshida, Makiko
AU - Yamana, Keiji
AU - Thwin, Khin Kyae Mon
AU - Kuroda, Jumpei
AU - Kurokawa, Daisuke
AU - Koda, Tsubasa
AU - Nishida, Kosuke
AU - Ikuta, Toshihiko
AU - Fujioka, Kazumichi
AU - Mizobuchi, Masami
AU - Taniguchi-Ikeda, Mariko
AU - Morioka, Ichiro
AU - Iijima, Kazumoto
AU - Nishimura, Noriyuki
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research (C) (Grant no. 25461644) and Young Scientists (B) (Grant no. 26860845) of JSPS KAKENHI. The authors thank Drs. Shohei Ohyama, Sachiyo Fukushima, Oshi Tokuda, Kaori Maeyama, and Miwako Nagasaka for collecting the umbilical cord samples.
Publisher Copyright:
© 2017 Sota Iwatani et al.
PY - 2017
Y1 - 2017
N2 - Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.
AB - Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.
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U2 - 10.1155/2017/8749751
DO - 10.1155/2017/8749751
M3 - Article
AN - SCOPUS:85030667271
SN - 1687-9678
VL - 2017
JO - Stem Cells International
JF - Stem Cells International
M1 - 8749751
ER -