Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation

Sota Iwatani, Akemi Shono, Makiko Yoshida, Keiji Yamana, Khin Kyae Mon Thwin, Jumpei Kuroda, Daisuke Kurokawa, Tsubasa Koda, Kosuke Nishida, Toshihiko Ikuta, Kazumichi Fujioka, Masami Mizobuchi, Mariko Ikeda, Ichiro Morioka, Kazumoto Iijima, Noriyuki Nishimura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.

Original languageEnglish
Article number8749751
JournalStem Cells International
Volume2017
DOIs
Publication statusPublished - 01-01-2017

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Umbilical Cord
Mesenchymal Stromal Cells
Gestational Age
Cell Proliferation
Cell Differentiation
Bone Marrow
Gene Expression
Microarray Analysis
Cell- and Tissue-Based Therapy
Genes
Stem Cells

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Iwatani, Sota ; Shono, Akemi ; Yoshida, Makiko ; Yamana, Keiji ; Thwin, Khin Kyae Mon ; Kuroda, Jumpei ; Kurokawa, Daisuke ; Koda, Tsubasa ; Nishida, Kosuke ; Ikuta, Toshihiko ; Fujioka, Kazumichi ; Mizobuchi, Masami ; Ikeda, Mariko ; Morioka, Ichiro ; Iijima, Kazumoto ; Nishimura, Noriyuki. / Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation. In: Stem Cells International. 2017 ; Vol. 2017.
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abstract = "Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.",
author = "Sota Iwatani and Akemi Shono and Makiko Yoshida and Keiji Yamana and Thwin, {Khin Kyae Mon} and Jumpei Kuroda and Daisuke Kurokawa and Tsubasa Koda and Kosuke Nishida and Toshihiko Ikuta and Kazumichi Fujioka and Masami Mizobuchi and Mariko Ikeda and Ichiro Morioka and Kazumoto Iijima and Noriyuki Nishimura",
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Iwatani, S, Shono, A, Yoshida, M, Yamana, K, Thwin, KKM, Kuroda, J, Kurokawa, D, Koda, T, Nishida, K, Ikuta, T, Fujioka, K, Mizobuchi, M, Ikeda, M, Morioka, I, Iijima, K & Nishimura, N 2017, 'Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation', Stem Cells International, vol. 2017, 8749751. https://doi.org/10.1155/2017/8749751

Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation. / Iwatani, Sota; Shono, Akemi; Yoshida, Makiko; Yamana, Keiji; Thwin, Khin Kyae Mon; Kuroda, Jumpei; Kurokawa, Daisuke; Koda, Tsubasa; Nishida, Kosuke; Ikuta, Toshihiko; Fujioka, Kazumichi; Mizobuchi, Masami; Ikeda, Mariko; Morioka, Ichiro; Iijima, Kazumoto; Nishimura, Noriyuki.

In: Stem Cells International, Vol. 2017, 8749751, 01.01.2017.

Research output: Contribution to journalArticle

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T1 - Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation

AU - Iwatani, Sota

AU - Shono, Akemi

AU - Yoshida, Makiko

AU - Yamana, Keiji

AU - Thwin, Khin Kyae Mon

AU - Kuroda, Jumpei

AU - Kurokawa, Daisuke

AU - Koda, Tsubasa

AU - Nishida, Kosuke

AU - Ikuta, Toshihiko

AU - Fujioka, Kazumichi

AU - Mizobuchi, Masami

AU - Ikeda, Mariko

AU - Morioka, Ichiro

AU - Iijima, Kazumoto

AU - Nishimura, Noriyuki

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.

AB - Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.

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