TY - JOUR
T1 - Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation
AU - Iwatani, Sota
AU - Shono, Akemi
AU - Yoshida, Makiko
AU - Yamana, Keiji
AU - Thwin, Khin Kyae Mon
AU - Kuroda, Jumpei
AU - Kurokawa, Daisuke
AU - Koda, Tsubasa
AU - Nishida, Kosuke
AU - Ikuta, Toshihiko
AU - Fujioka, Kazumichi
AU - Mizobuchi, Masami
AU - Taniguchi-Ikeda, Mariko
AU - Morioka, Ichiro
AU - Iijima, Kazumoto
AU - Nishimura, Noriyuki
N1 - Publisher Copyright:
© 2017 Sota Iwatani et al.
PY - 2017
Y1 - 2017
N2 - Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.
AB - Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.
UR - https://www.scopus.com/pages/publications/85030667271
UR - https://www.scopus.com/pages/publications/85030667271#tab=citedBy
U2 - 10.1155/2017/8749751
DO - 10.1155/2017/8749751
M3 - Article
AN - SCOPUS:85030667271
SN - 1687-966X
VL - 2017
JO - Stem Cells International
JF - Stem Cells International
M1 - 8749751
ER -