IQGAP3, a novel effector of Rac1 and Cdc42, regulates neurite outgrowth

Shujie Wang, Takashi Watanabe, Jun Noritake, Masaki Fukuta, Takeshi Yoshimura, Norimichi Itoh, Takumi Harada, Masato Nakagawa, Yoshiharu Matsuura, Nariko Arimura, Kozo Kaibuchi

Research output: Contribution to journalArticlepeer-review

102 Citations (Scopus)

Abstract

Rac1 Rand Cdc42, members of the Rho family GTPases, control diverse cellular processes such as cell migration and morphogenesis through their effectors. Among the effectors, IQGAP1 plays pivotal roles in the establishment of cytoskeletal architecture and intercellular adhesions in various cells. However, its roles remain to be clarified, especially in neuronal cells. We have identified IQGAP3 as a novel member of the IQGAP family, which is highly expressed in brain. We found that IQGAP3, an effector of Rac1 and Cdc42, associates directly with actin filaments and accumulates asymmetrically at the distal region of axons in hippocampal neurons. The depletion of IQGAP3 impairs neurite or axon outgrowth in neuronal cells with the disorganized cytoskeleton, but depletion of IQGAP1 does not. Furthermore, IQGAP3 is indispensable for Rac1/Cdc42-promoted neurite outgrowth in PC12 cells. Taken together, these results indicate that IQGAP3 can link the activation of Rac1 and Cdc42 with the cytoskeletal architectures during neuronal morphogenesis.

Original languageEnglish
Pages (from-to)567-577
Number of pages11
JournalJournal of cell science
Volume120
Issue number4
DOIs
Publication statusPublished - 15-02-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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