TY - JOUR
T1 - Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons
AU - Hiramatsu, Noriko
AU - Yamamoto, Naoki
AU - Ohkuma, Mahito
AU - Nagai, Noriaki
AU - Miyachi, Ei ichi
AU - Yamatsuta, Kumiko
AU - Imaizumi, Kazuyoshi
N1 - Publisher Copyright:
© 2022, The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.
PY - 2022/12
Y1 - 2022/12
N2 - When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.
AB - When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.
KW - Electrophysiology
KW - Green fluorescent protein
KW - Mouse iris-derived induced pluripotent stem cells
KW - Neurons
UR - https://www.scopus.com/pages/publications/85135695411
UR - https://www.scopus.com/pages/publications/85135695411#tab=citedBy
U2 - 10.1007/s00795-022-00330-z
DO - 10.1007/s00795-022-00330-z
M3 - Article
C2 - 35932315
AN - SCOPUS:85135695411
SN - 1860-1480
VL - 55
SP - 292
EP - 303
JO - Medical Molecular Morphology
JF - Medical Molecular Morphology
IS - 4
ER -