Abstract
When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.
| Original language | English |
|---|---|
| Pages (from-to) | 292-303 |
| Number of pages | 12 |
| Journal | Medical Molecular Morphology |
| Volume | 55 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 12-2022 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Molecular Biology
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