IRR is involved in glucose-induced endocytosis after insulin secretion

Mami Yamaoka; Takeshi Terabayashi; Tomoki Nishioka; Kozo Kaibuchi; Tomohisa Ishikawa; Toshimasa Ishizaki; Toshihide Kimura

doi:10.1016/j.jphs.2019.07.002

IRR is involved in glucose-induced endocytosis after insulin secretion

Mami Yamaoka, Takeshi Terabayashi, Tomoki Nishioka, Kozo Kaibuchi, Tomohisa Ishikawa, Toshimasa Ishizaki, Toshihide Kimura

Institute for Comprehensive Medical Science

Research output: Contribution to journal › Article

Abstract

Endocytosis after insulin secretion plays a pivotal role in the regulation of insulin secretion in pancreatic β-cells. Our recent study suggested that EPI64, a GTPase activating protein for Rab27a, contributes to the regulation of glucose-induced endocytosis, which is mediated by the GDP-bound form of Rab27a. Here, we identified insulin receptor-related receptor (IRR) as an EPI64-interacting protein. Knockdown of IRR inhibited glucose-induced uptake of transferrin, a marker of endocytosis, translocation of the guanine-nucleotide-exchange factor ARNO to the plasma membrane, and generation of phosphatidylinositol 3,4,5-trisphosphate (PIP₃). These results suggest that IRR functions upstream of PIP₃ generation and controls endocytosis after insulin secretion.

Original language	English
Pages (from-to)	300-304
Number of pages	5
Journal	Journal of Pharmacological Sciences
Volume	140
Issue number	3
DOIs	https://doi.org/10.1016/j.jphs.2019.07.002
Publication status	Published - 07-2019

All Science Journal Classification (ASJC) codes

Molecular Medicine
Pharmacology

Access to Document

10.1016/j.jphs.2019.07.002

Fingerprint Dive into the research topics of 'IRR is involved in glucose-induced endocytosis after insulin secretion'. Together they form a unique fingerprint.

Cite this

Yamaoka, M., Terabayashi, T., Nishioka, T., Kaibuchi, K., Ishikawa, T., Ishizaki, T., & Kimura, T. (2019). IRR is involved in glucose-induced endocytosis after insulin secretion. Journal of Pharmacological Sciences, 140(3), 300-304. https://doi.org/10.1016/j.jphs.2019.07.002

@article{85f3d10ab64443b48c0e68c96cf718d1,

title = "IRR is involved in glucose-induced endocytosis after insulin secretion",

abstract = "Endocytosis after insulin secretion plays a pivotal role in the regulation of insulin secretion in pancreatic β-cells. Our recent study suggested that EPI64, a GTPase activating protein for Rab27a, contributes to the regulation of glucose-induced endocytosis, which is mediated by the GDP-bound form of Rab27a. Here, we identified insulin receptor-related receptor (IRR) as an EPI64-interacting protein. Knockdown of IRR inhibited glucose-induced uptake of transferrin, a marker of endocytosis, translocation of the guanine-nucleotide-exchange factor ARNO to the plasma membrane, and generation of phosphatidylinositol 3,4,5-trisphosphate (PIP3). These results suggest that IRR functions upstream of PIP3 generation and controls endocytosis after insulin secretion.",

author = "Mami Yamaoka and Takeshi Terabayashi and Tomoki Nishioka and Kozo Kaibuchi and Tomohisa Ishikawa and Toshimasa Ishizaki and Toshihide Kimura",

year = "2019",

month = jul,

doi = "10.1016/j.jphs.2019.07.002",

language = "English",

volume = "140",

pages = "300--304",

journal = "Journal of Pharmacological Sciences",

issn = "1347-8613",

publisher = "Japanese Pharmacological Society",

number = "3",

}

TY - JOUR

T1 - IRR is involved in glucose-induced endocytosis after insulin secretion

AU - Yamaoka, Mami

AU - Terabayashi, Takeshi

AU - Nishioka, Tomoki

AU - Kaibuchi, Kozo

AU - Ishikawa, Tomohisa

AU - Ishizaki, Toshimasa

AU - Kimura, Toshihide

PY - 2019/7

Y1 - 2019/7

N2 - Endocytosis after insulin secretion plays a pivotal role in the regulation of insulin secretion in pancreatic β-cells. Our recent study suggested that EPI64, a GTPase activating protein for Rab27a, contributes to the regulation of glucose-induced endocytosis, which is mediated by the GDP-bound form of Rab27a. Here, we identified insulin receptor-related receptor (IRR) as an EPI64-interacting protein. Knockdown of IRR inhibited glucose-induced uptake of transferrin, a marker of endocytosis, translocation of the guanine-nucleotide-exchange factor ARNO to the plasma membrane, and generation of phosphatidylinositol 3,4,5-trisphosphate (PIP3). These results suggest that IRR functions upstream of PIP3 generation and controls endocytosis after insulin secretion.

AB - Endocytosis after insulin secretion plays a pivotal role in the regulation of insulin secretion in pancreatic β-cells. Our recent study suggested that EPI64, a GTPase activating protein for Rab27a, contributes to the regulation of glucose-induced endocytosis, which is mediated by the GDP-bound form of Rab27a. Here, we identified insulin receptor-related receptor (IRR) as an EPI64-interacting protein. Knockdown of IRR inhibited glucose-induced uptake of transferrin, a marker of endocytosis, translocation of the guanine-nucleotide-exchange factor ARNO to the plasma membrane, and generation of phosphatidylinositol 3,4,5-trisphosphate (PIP3). These results suggest that IRR functions upstream of PIP3 generation and controls endocytosis after insulin secretion.

UR - http://www.scopus.com/inward/record.url?scp=85071380833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071380833&partnerID=8YFLogxK

U2 - 10.1016/j.jphs.2019.07.002

DO - 10.1016/j.jphs.2019.07.002

M3 - Article

C2 - 31353211

AN - SCOPUS:85071380833

VL - 140

SP - 300

EP - 304

JO - Journal of Pharmacological Sciences

JF - Journal of Pharmacological Sciences

SN - 1347-8613

IS - 3

ER -