Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells

Koji Ohira, Takahiro Furuta, Hiroyuki Hioki, Kouichi C. Nakamura, Eriko Kuramoto, Yasuyo Tanaka, Nobuo Funatsu, Keiko Shimizu, Takao Oishi, Motoharu Hayashi, Tsuyoshi Miyakawa, Takeshi Kaneko, Shun Nakamura

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Adult mammalian neurogenesis occurs in the hippocampus and the olfactory bulb, whereas neocortical adult neurogenesis remains controversial. Several occurrences of neocortical adult neurogenesis in injured neocortex were recently reported, suggesting that neural stem cells (NSCs) or neuronal progenitor cells (NPCs) that can be activated by injury are maintained in the adult brain. However, it is not clear whether or where neocortical NSCs/NPCs exist in the brain. We found NPCs in the neocortical layer 1 of adult rats and observed that their proliferation was highly activated by global forebrain ischemia. Using retrovirus-mediated labeling of layer 1 proliferating cells with membrane-targeted green fluorescent protein, we found that the newly generated neurons were GABAergic and that the neurons were functionally integrated into the neuronal circuitry. Our results suggest that layer 1 NPCs are a source of adult neurogenesis under ischemic conditions.

Original languageEnglish
Pages (from-to)173-179
Number of pages7
JournalNature Neuroscience
Volume13
Issue number2
DOIs
Publication statusPublished - 18-02-2010

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Neurogenesis
Stem Cells
Ischemia
Neural Stem Cells
GABAergic Neurons
Olfactory Bulb
Neocortex
Brain
Retroviridae
Prosencephalon
Green Fluorescent Proteins
Hippocampus
Cell Membrane
Neurons
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Ohira, K., Furuta, T., Hioki, H., Nakamura, K. C., Kuramoto, E., Tanaka, Y., ... Nakamura, S. (2010). Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. Nature Neuroscience, 13(2), 173-179. https://doi.org/10.1038/nn.2473
Ohira, Koji ; Furuta, Takahiro ; Hioki, Hiroyuki ; Nakamura, Kouichi C. ; Kuramoto, Eriko ; Tanaka, Yasuyo ; Funatsu, Nobuo ; Shimizu, Keiko ; Oishi, Takao ; Hayashi, Motoharu ; Miyakawa, Tsuyoshi ; Kaneko, Takeshi ; Nakamura, Shun. / Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. In: Nature Neuroscience. 2010 ; Vol. 13, No. 2. pp. 173-179.
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Ohira, K, Furuta, T, Hioki, H, Nakamura, KC, Kuramoto, E, Tanaka, Y, Funatsu, N, Shimizu, K, Oishi, T, Hayashi, M, Miyakawa, T, Kaneko, T & Nakamura, S 2010, 'Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells', Nature Neuroscience, vol. 13, no. 2, pp. 173-179. https://doi.org/10.1038/nn.2473

Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. / Ohira, Koji; Furuta, Takahiro; Hioki, Hiroyuki; Nakamura, Kouichi C.; Kuramoto, Eriko; Tanaka, Yasuyo; Funatsu, Nobuo; Shimizu, Keiko; Oishi, Takao; Hayashi, Motoharu; Miyakawa, Tsuyoshi; Kaneko, Takeshi; Nakamura, Shun.

In: Nature Neuroscience, Vol. 13, No. 2, 18.02.2010, p. 173-179.

Research output: Contribution to journalArticle

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Ohira K, Furuta T, Hioki H, Nakamura KC, Kuramoto E, Tanaka Y et al. Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. Nature Neuroscience. 2010 Feb 18;13(2):173-179. https://doi.org/10.1038/nn.2473