Isoforms of caveolin-1 and caveolar structure

T. Fujimoto, H. Kogo, Ryuji Nomura, T. Une

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

The relationship between caveolin-1 isoforms (α and β) and caveolar ultrastructure was studied. By immunofluorescence microscopy of human fibroblasts, caveolae were observed as dots positive for caveolin-1, but many dots labeled by an antibody recognizing both isoforms (anti-αβ) were not labeled by another antibody specific for the α isoform (anti-α). Immunogold electron microscopy of freeze-fracture replicas revealed caveolae of different depths, and indicated that anti-α labeled deep caveolae preferentially over shallow ones, whereas anti-αβ labeled both forms with an equivalent frequency and intensity. The presence of the β isoform in deep caveolae was confirmed by labeling epitope-tagged β-caveolin. When made to be expressed in HepG2 cells lacking endogenous caveolins, the α isoform formed caveolar depressions efficiently, but the β isoform hardly did so. Caveolae were also formed in cells expressing the two isoforms, but their frequency was variable among cells of the same clone. Coexpression of caveolin-1 and caveolin-2 caused more efficient formation of deep caveolae than caveolin-1 alone. The result indicates that the two isoforms of caveolin-1 have a different potential for forming caveolae structure, and more importantly, that deep and shallow caveolae may be diversified in their molecular composition.

Original languageEnglish
Pages (from-to)3509-3517
Number of pages9
JournalJournal of Cell Science
Volume113
Issue number19
Publication statusPublished - 23-11-2000

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Caveolin 1
Caveolae
Protein Isoforms
Caveolins
Caveolin 2
Antibodies
Hep G2 Cells
Fluorescence Microscopy
Epitopes
Electron Microscopy
Clone Cells
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Fujimoto, T., Kogo, H., Nomura, R., & Une, T. (2000). Isoforms of caveolin-1 and caveolar structure. Journal of Cell Science, 113(19), 3509-3517.
Fujimoto, T. ; Kogo, H. ; Nomura, Ryuji ; Une, T. / Isoforms of caveolin-1 and caveolar structure. In: Journal of Cell Science. 2000 ; Vol. 113, No. 19. pp. 3509-3517.
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Fujimoto, T, Kogo, H, Nomura, R & Une, T 2000, 'Isoforms of caveolin-1 and caveolar structure', Journal of Cell Science, vol. 113, no. 19, pp. 3509-3517.

Isoforms of caveolin-1 and caveolar structure. / Fujimoto, T.; Kogo, H.; Nomura, Ryuji; Une, T.

In: Journal of Cell Science, Vol. 113, No. 19, 23.11.2000, p. 3509-3517.

Research output: Contribution to journalArticle

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Fujimoto T, Kogo H, Nomura R, Une T. Isoforms of caveolin-1 and caveolar structure. Journal of Cell Science. 2000 Nov 23;113(19):3509-3517.