Isolation and expression of a human SRY-related cDNA, hSOX20

Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Koji Taniguchi; Takuma Fujii; Akihiro Umezawa; Jun Ichi Hata; Sadakazu Aiso

doi:10.1016/S0167-4781(97)00186-3

Isolation and expression of a human SRY-related cDNA, hSOX20

Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Koji Taniguchi, Takuma Fujii, Akihiro Umezawa, Jun Ichi Hata, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

Original language	English
Pages (from-to)	132-137
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1396
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(97)00186-3
Publication status	Published - 09-03-1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(97)00186-3

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title = "Isolation and expression of a human SRY-related cDNA, hSOX20",

abstract = "SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.",

author = "Yoshiki Hiraoka and Motoyuki Ogawa and Yukinao Sakai and Koji Taniguchi and Takuma Fujii and Akihiro Umezawa and Hata, {Jun Ichi} and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Minstry of Education, Science and Culture to S. A. and Grants-in Aids from Ministry of Education, Science and Culture to S. A. and Y. H., and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.",

year = "1998",

month = mar,

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doi = "10.1016/S0167-4781(97)00186-3",

language = "English",

volume = "1396",

pages = "132--137",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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TY - JOUR

T1 - Isolation and expression of a human SRY-related cDNA, hSOX20

AU - Hiraoka, Yoshiki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Taniguchi, Koji

AU - Fujii, Takuma

AU - Umezawa, Akihiro

AU - Hata, Jun Ichi

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Minstry of Education, Science and Culture to S. A. and Grants-in Aids from Ministry of Education, Science and Culture to S. A. and Y. H., and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.

PY - 1998/3/9

Y1 - 1998/3/9

N2 - SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

AB - SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

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DO - 10.1016/S0167-4781(97)00186-3

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AN - SCOPUS:0032498831

SN - 0167-4781

VL - 1396

SP - 132

EP - 137

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 2

ER -

Isolation and expression of a human SRY-related cDNA, hSOX20

Abstract

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