Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin

Mai Kakita; Tsuyoshi Takahashi; Takako Komiya; Yoshitaka Iba; Takao Tsuji; Yoshikazu Kurosawa; Motohide Takahashi

doi:10.1128/IAI.01731-05

Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin

Mai Kakita
, Tsuyoshi Takahashi
, Takako Komiya
, Yoshitaka Iba
, Takao Tsuji
, Yoshikazu Kurosawa
, Motohide Takahashi

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (K_d) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.

Original language	English
Pages (from-to)	3682-3683
Number of pages	2
Journal	Infection and Immunity
Volume	74
Issue number	6
DOIs	https://doi.org/10.1128/IAI.01731-05
Publication status	Published - 06-2006
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Parasitology
Microbiology
Immunology
Infectious Diseases

Access to Document

10.1128/IAI.01731-05

Cite this

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title = "Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin",

abstract = "We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.",

author = "Mai Kakita and Tsuyoshi Takahashi and Takako Komiya and Yoshitaka Iba and Takao Tsuji and Yoshikazu Kurosawa and Motohide Takahashi",

note = "Funding Information: The assistance of residents of the Tahuayo, Amazon, Ucayali, Putumayo, and Nanay Rivers is gratefully acknowledged. Special thanks go to colleagues and staff at IIAP-Iquitos, IIAP-Pucallpa, INIA-Iquitos, PNCC-Iquitos, PNCCPucallpa, INADE-Iquitos, INRENA-El Estrecho, and INPA-Manaus. Mario Pinedo, Jorge Soplin, Dennis Huayllahua and Pilar Diaz are acknowledged for their exceptional support and collaboration. Field research was generously funded by the Rainforest Conservation Fund (RCF) of Chicago, Illinois; the Dallas Zoological Society, and the Tropical Conservation and Development Program of the University of Florida.",

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month = jun,

doi = "10.1128/IAI.01731-05",

language = "English",

volume = "74",

pages = "3682--3683",

journal = "Infection and Immunity",

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T1 - Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin

AU - Kakita, Mai

AU - Takahashi, Tsuyoshi

AU - Komiya, Takako

AU - Iba, Yoshitaka

AU - Tsuji, Takao

AU - Kurosawa, Yoshikazu

AU - Takahashi, Motohide

N1 - Funding Information: The assistance of residents of the Tahuayo, Amazon, Ucayali, Putumayo, and Nanay Rivers is gratefully acknowledged. Special thanks go to colleagues and staff at IIAP-Iquitos, IIAP-Pucallpa, INIA-Iquitos, PNCC-Iquitos, PNCCPucallpa, INADE-Iquitos, INRENA-El Estrecho, and INPA-Manaus. Mario Pinedo, Jorge Soplin, Dennis Huayllahua and Pilar Diaz are acknowledged for their exceptional support and collaboration. Field research was generously funded by the Rainforest Conservation Fund (RCF) of Chicago, Illinois; the Dallas Zoological Society, and the Tropical Conservation and Development Program of the University of Florida.

PY - 2006/6

Y1 - 2006/6

N2 - We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.

AB - We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.

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U2 - 10.1128/IAI.01731-05

DO - 10.1128/IAI.01731-05

M3 - Article

C2 - 16714603

AN - SCOPUS:33646948222

SN - 0019-9567

VL - 74

SP - 3682

EP - 3683

JO - Infection and Immunity

JF - Infection and Immunity

IS - 6

ER -

Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin

Abstract

UN SDGs

All Science Journal Classification (ASJC) codes

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