TY - JOUR
T1 - Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents
AU - Teye, Kwesi
AU - Numata, Sanae
AU - Ishii, Norito
AU - Krol, Rafal P.
AU - Tsuchisaka, Atsunari
AU - Hamada, Takahiro
AU - Koga, Hiroshi
AU - Karashima, Tadashi
AU - Ohata, Chika
AU - Tsuruta, Daisuke
AU - Saya, Hideyuki
AU - Haftek, Marek
AU - Hashimoto, Takashi
N1 - Funding Information:
This study was supported by Grants-in-Aid for Scientific Research (grant numbers 26860904), and Supported Program for the Strategic Research Foundation at Private Universities from the Ministry of Education, Culture, Sports, Science and Technology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We gratefully appreciate Ms. Tomoko Tashima, Ms. Ms. Chiharu Yoshida, Ms. Naomi Iwasa and Ms. Miwa Shiramizu for their secretarial work. We thank Dr. N.E. Fusenig (University of Ulm, Ulm, Germany), Dr. Y. Kitajima (Department of Dermatology, Kizawa Memorial Hospital, Gifu, Japan), and T. Tsukamoto (Department of Urology, School of Medicine, Keio University, Tokyo, Japan) for kindly providing us with their cell lines.
PY - 2016/8
Y1 - 2016/8
N2 - CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.
AB - CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.
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U2 - 10.1371/journal.pone.0160952
DO - 10.1371/journal.pone.0160952
M3 - Article
C2 - 27505250
AN - SCOPUS:84983739119
VL - 11
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 8
M1 - e0160952
ER -