Isolation of carp genes encoding major histocompatibility complex antigens

K. Hashimoto, T. Nakanishi, Y. Kurosawa

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

In the evolution of the adaptive immune system unique to vertebrates, teleost fish occupy the critical position. This is the most primitive class of lower vertebrates in which the capacity for acute allograft rejections can be demonstrated, thus suggesting the presence of major histocompatibility complex (MHC) antigens and, therefore, T cells. Here, we report the identification of two putative MHC-antigen-encoding sequences in the carp Cyprinus carpio. One, identified as TLAIα-1, had reasonable homology to MHC class I heavy chains of mammalian and avian species, while the other, identified as TLAIIβ-1, was homologous to MHC class II β chain of the aforementioned higher vertebrates. For these isolations of fish MHC genes, we have identified two highly conserved amino acid sequence blocks surrounding two cysteine residues in the second domain of MHC class II β chains as well as the third domain of class I heavy chains of humans, mice, and chickens. Two kinds of mixed oligonucleotide probes corresponding to these two regions were synthesized. The carp genomic DNA was subjected to amplification by polymerase chain reaction using the above two synthetic DNA fragments as primers. Subsequently, two different DNA sequences sandwiched by these primers were isolated from the amplified products. Their use as secondary probes led to the identification of TLAIα-1 and TLAIIβ-1. We also discuss the applicability of the above approach for isolation from lower vertebrates of other genes belonging to the immunoglobulin superfamily as well as the evolutionary origin of vertebrate MHC antigens.

Original languageEnglish
Pages (from-to)6863-6867
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number17
DOIs
Publication statusPublished - 01-01-1990

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Histocompatibility Antigens
Carps
Major Histocompatibility Complex
Vertebrates
Genes
Fishes
Oligonucleotide Probes
DNA
Allografts
Cysteine
Immunoglobulins
Amino Acid Sequence
Chickens
Immune System
T-Lymphocytes
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Isolation of carp genes encoding major histocompatibility complex antigens",
abstract = "In the evolution of the adaptive immune system unique to vertebrates, teleost fish occupy the critical position. This is the most primitive class of lower vertebrates in which the capacity for acute allograft rejections can be demonstrated, thus suggesting the presence of major histocompatibility complex (MHC) antigens and, therefore, T cells. Here, we report the identification of two putative MHC-antigen-encoding sequences in the carp Cyprinus carpio. One, identified as TLAIα-1, had reasonable homology to MHC class I heavy chains of mammalian and avian species, while the other, identified as TLAIIβ-1, was homologous to MHC class II β chain of the aforementioned higher vertebrates. For these isolations of fish MHC genes, we have identified two highly conserved amino acid sequence blocks surrounding two cysteine residues in the second domain of MHC class II β chains as well as the third domain of class I heavy chains of humans, mice, and chickens. Two kinds of mixed oligonucleotide probes corresponding to these two regions were synthesized. The carp genomic DNA was subjected to amplification by polymerase chain reaction using the above two synthetic DNA fragments as primers. Subsequently, two different DNA sequences sandwiched by these primers were isolated from the amplified products. Their use as secondary probes led to the identification of TLAIα-1 and TLAIIβ-1. We also discuss the applicability of the above approach for isolation from lower vertebrates of other genes belonging to the immunoglobulin superfamily as well as the evolutionary origin of vertebrate MHC antigens.",
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Isolation of carp genes encoding major histocompatibility complex antigens. / Hashimoto, K.; Nakanishi, T.; Kurosawa, Y.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 17, 01.01.1990, p. 6863-6867.

Research output: Contribution to journalArticle

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