TY - JOUR
T1 - Isolation of mesenchymal stromal/stem cells from small-volume umbilical cord blood units that do not qualify for the banking system
AU - Yoshioka, Satoshi
AU - Miura, Yasuo
AU - Iwasa, Masaki
AU - Fujishiro, Aya
AU - Yao, Hisayuki
AU - Miura, Masako
AU - Fukuoka, Masaaki
AU - Nakagawa, Yoko
AU - Yokota, Asumi
AU - Hirai, Hideyo
AU - Ichinohe, Tatsuo
AU - Takaori-Kondo, Akifumi
AU - Maekawa, Taira
N1 - Publisher Copyright:
© 2015, The Japanese Society of Hematology.
PY - 2015/8/11
Y1 - 2015/8/11
N2 - The clinical application of mesenchymal stromal/stem cells (MSCs) has been extensively explored. In this study, we examined the availability of freshly donated umbilical cord blood (UCB) units that do not qualify for the Japanese banking system for transplantation because of their small volume as a source of MSCs. Forty-five UCB units were used. The median volume of each UCB unit and number of nucleated cells per unit were 40 mL and 5.39 × 108, respectively. MSCs were successfully isolated from 18 of 45 units (40 %). The MSC isolation rate was not affected by cell processing method or the interval between delivery and cell processing. The volume of the UCB unit and the mononuclear cell count were predictive factors of the MSC isolation rate. MSCs were effectively isolated by selecting UCB units with a volume of ≥54 mL and containing ≥1.28 × 108 mononuclear cells, yielding a MSC isolation rate of >70 %. UCB-derived MSCs were similar to bone marrow-derived MSCs in terms of their morphology, surface marker expression, and differentiation potential, apart from adipogenesis. Our data indicate that UCB units that are currently discarded due to inadequate volume should be reconsidered as a source of MSCs using the well-established UCB banking system.
AB - The clinical application of mesenchymal stromal/stem cells (MSCs) has been extensively explored. In this study, we examined the availability of freshly donated umbilical cord blood (UCB) units that do not qualify for the Japanese banking system for transplantation because of their small volume as a source of MSCs. Forty-five UCB units were used. The median volume of each UCB unit and number of nucleated cells per unit were 40 mL and 5.39 × 108, respectively. MSCs were successfully isolated from 18 of 45 units (40 %). The MSC isolation rate was not affected by cell processing method or the interval between delivery and cell processing. The volume of the UCB unit and the mononuclear cell count were predictive factors of the MSC isolation rate. MSCs were effectively isolated by selecting UCB units with a volume of ≥54 mL and containing ≥1.28 × 108 mononuclear cells, yielding a MSC isolation rate of >70 %. UCB-derived MSCs were similar to bone marrow-derived MSCs in terms of their morphology, surface marker expression, and differentiation potential, apart from adipogenesis. Our data indicate that UCB units that are currently discarded due to inadequate volume should be reconsidered as a source of MSCs using the well-established UCB banking system.
UR - http://www.scopus.com/inward/record.url?scp=84938982987&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938982987&partnerID=8YFLogxK
U2 - 10.1007/s12185-015-1828-7
DO - 10.1007/s12185-015-1828-7
M3 - Article
C2 - 26121953
AN - SCOPUS:84938982987
SN - 0925-5710
VL - 102
SP - 218
EP - 229
JO - International Journal of Hematology
JF - International Journal of Hematology
IS - 2
ER -