Isolation of the Complementary DNA Encoding a Mouse Heparin-binding Growth Factor Receptor with the Use of a Unique Kinase Insert Sequence

Osamu Katoh, Yutaka Hattori, Hiroki Sasaki, Hiromi Sakamoto, Kiyohide Fujimoto, Takuma Fujii, Takashi Sugimura, Masaaki Terada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

With the use of reverse transcriptase-polymerase chain reaction techniques focused on a unique kinase insert sequence, the complementary DNA for a mouse tyrosine kinase receptor gene, designated sam3> was isolated from a mouse brain complementary DNA library as a member of the heparin-binding growth factor receptor family or fibroblast growth factor receptor family. The kinase insert region was selected as the probe synthesized by polymerase chain reaction techniques because it composes a unique structure in this receptor family. The sam3 protein, 800 amino acids long, has high homology to mouse K-sam/bek (67%) and N-sam/flg (63%), which we also cloned as the mouse counterparts of human K-sam/ bek and N-sam/flg genes, other members of this family. The sam3 protein also has high homology to human FGFR3 (92%) and chicken cek2 (80%) proteins. The sam3 protein is most likely to be a mouse counterpart of human FGFR3 and chicken cek2 proteins. mRNAs of K-samlbek, N-sam/ flg, and sam3/FGFR3 genes were detected in mouse embryo through some adult tissues. The relative amounts of these mRNAs were different depending on the organs examined. Thus, these gene products may have different biological functions in organ development including the central nervous system.

Original languageEnglish
Pages (from-to)1136-1141
Number of pages6
JournalCancer Research
Volume53
Issue number5
Publication statusPublished - 01-01-1993
Externally publishedYes

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Fibroblast Growth Factor Receptors
Phosphotransferases
Complementary DNA
Proteins
Genes
Chickens
Messenger RNA
Receptor Protein-Tyrosine Kinases
Reverse Transcriptase Polymerase Chain Reaction
Gene Library
Embryonic Structures
Central Nervous System
Short Stature, Auditory Canal Atresia, Mandibular Hypoplasia, Skeletal Abnormalities
Amino Acids
Polymerase Chain Reaction
Brain

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Katoh, Osamu ; Hattori, Yutaka ; Sasaki, Hiroki ; Sakamoto, Hiromi ; Fujimoto, Kiyohide ; Fujii, Takuma ; Sugimura, Takashi ; Terada, Masaaki. / Isolation of the Complementary DNA Encoding a Mouse Heparin-binding Growth Factor Receptor with the Use of a Unique Kinase Insert Sequence. In: Cancer Research. 1993 ; Vol. 53, No. 5. pp. 1136-1141.
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abstract = "With the use of reverse transcriptase-polymerase chain reaction techniques focused on a unique kinase insert sequence, the complementary DNA for a mouse tyrosine kinase receptor gene, designated sam3> was isolated from a mouse brain complementary DNA library as a member of the heparin-binding growth factor receptor family or fibroblast growth factor receptor family. The kinase insert region was selected as the probe synthesized by polymerase chain reaction techniques because it composes a unique structure in this receptor family. The sam3 protein, 800 amino acids long, has high homology to mouse K-sam/bek (67{\%}) and N-sam/flg (63{\%}), which we also cloned as the mouse counterparts of human K-sam/ bek and N-sam/flg genes, other members of this family. The sam3 protein also has high homology to human FGFR3 (92{\%}) and chicken cek2 (80{\%}) proteins. The sam3 protein is most likely to be a mouse counterpart of human FGFR3 and chicken cek2 proteins. mRNAs of K-samlbek, N-sam/ flg, and sam3/FGFR3 genes were detected in mouse embryo through some adult tissues. The relative amounts of these mRNAs were different depending on the organs examined. Thus, these gene products may have different biological functions in organ development including the central nervous system.",
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Katoh, O, Hattori, Y, Sasaki, H, Sakamoto, H, Fujimoto, K, Fujii, T, Sugimura, T & Terada, M 1993, 'Isolation of the Complementary DNA Encoding a Mouse Heparin-binding Growth Factor Receptor with the Use of a Unique Kinase Insert Sequence', Cancer Research, vol. 53, no. 5, pp. 1136-1141.

Isolation of the Complementary DNA Encoding a Mouse Heparin-binding Growth Factor Receptor with the Use of a Unique Kinase Insert Sequence. / Katoh, Osamu; Hattori, Yutaka; Sasaki, Hiroki; Sakamoto, Hiromi; Fujimoto, Kiyohide; Fujii, Takuma; Sugimura, Takashi; Terada, Masaaki.

In: Cancer Research, Vol. 53, No. 5, 01.01.1993, p. 1136-1141.

Research output: Contribution to journalArticle

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