TY - JOUR
T1 - Isolation of the interacting molecules with GEX-3 by a novel functional screening
AU - Tsuboi, Daisuke
AU - Qadota, Hiroshi
AU - Kasuya, Katsuhisa
AU - Amano, Mutsuki
AU - Kaibuchi, Kozo
N1 - Funding Information:
This work was supported by grants-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, by Research for the Future Program from Japan Society for the Promotion of Science, and by a grant from Human Frontier Science Program Organization. We thank Drs. Craig C. Mello and Martha C. Soto (University of Massachusetts) for the evaluating RNAi, Dr. Robert J. Barstead (Oklahoma Medical Research Foundation) and Dr. Ken-ichi Kariya (University of Ryukyus) for providing the C. elegans cDNA library, Dr. Jeffrey D. Hardin and Dr. Jeffrey S. Simske (University of Wisconsin–Madison) for providing SU93 strain. Some of the strains used in this work were provided by the Caenorhabditis elegans Genetics Center, which is funded by the National Institutes of Health (NIH) Center for Research Resources, Dr. Ken Norman (University of British Columbia) for critical reading of the manuscript, and Dr. Shinya Kuroda (University of Tokyo) and THE TEAM for valuable discussion. We are grateful to Takako Ishii for secretarial assistance.
PY - 2002
Y1 - 2002
N2 - To screen for important molecules that interact with a gene of interest in Caenorhabditis elegans (C. elegans), we established a novel functional screening system using the yeast two-hybrid system with the RNA interference technique. Our screening system makes it possible to identify the molecular machinery involved in the function of a gene of interest starting with the cDNA of this gene. As a model case, we examined the molecular machinery involved in the function of GEX-3, an essential factor of tissue morphogenesis. We identified many interacting molecules by yeast two-hybrid screening and could detect some functional interactions using this novel functional screening system.
AB - To screen for important molecules that interact with a gene of interest in Caenorhabditis elegans (C. elegans), we established a novel functional screening system using the yeast two-hybrid system with the RNA interference technique. Our screening system makes it possible to identify the molecular machinery involved in the function of a gene of interest starting with the cDNA of this gene. As a model case, we examined the molecular machinery involved in the function of GEX-3, an essential factor of tissue morphogenesis. We identified many interacting molecules by yeast two-hybrid screening and could detect some functional interactions using this novel functional screening system.
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U2 - 10.1006/bbrc.2002.6717
DO - 10.1006/bbrc.2002.6717
M3 - Article
C2 - 11922622
AN - SCOPUS:0036086109
SN - 0006-291X
VL - 292
SP - 697
EP - 701
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -