Isolation of the interacting molecules with GEX-3 by a novel functional screening

Daisuke Tsuboi, Hiroshi Qadota, Katsuhisa Kasuya, Mutsuki Amano, Kozo Kaibuchi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

To screen for important molecules that interact with a gene of interest in Caenorhabditis elegans (C. elegans), we established a novel functional screening system using the yeast two-hybrid system with the RNA interference technique. Our screening system makes it possible to identify the molecular machinery involved in the function of a gene of interest starting with the cDNA of this gene. As a model case, we examined the molecular machinery involved in the function of GEX-3, an essential factor of tissue morphogenesis. We identified many interacting molecules by yeast two-hybrid screening and could detect some functional interactions using this novel functional screening system.

Original languageEnglish
Pages (from-to)697-701
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume292
Issue number3
DOIs
Publication statusPublished - 2002

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Isolation of the interacting molecules with GEX-3 by a novel functional screening'. Together they form a unique fingerprint.

  • Cite this