ITRAQ-based proteomics reveals novel biomarkers of osteoarthritis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: We performed comprehensive proteomic analyses of articular cartilage by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and searched for candidate biomarkers for osteoarthritis (OA). Methods: Articular cartilage was collected from patients with OA or femoral neck fracture for the control group. Molecular variations were detected by the iTRAQ method, and quantitative analyses were performed by western blot. Results: Using the iTRAQ method, we identified 76 proteins with different expression levels in OA patients and the control group. Among these proteins, we selected LECT2 (leukocyte cell-derived chemotaxin-2), BAALC (brain and acute leukemia, cytoplasmic), and PRDX6 (peroxiredoxin-6), which had not been reported as biomarkers for OA. Conclusions: Use of these proteins in combination with conventional OA biomarkers may better reflect the grade and prognosis of OA.

Original languageEnglish
Pages (from-to)565-572
Number of pages8
JournalBiomarkers
Volume18
Issue number7
DOIs
Publication statusPublished - 01-11-2013

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Biomarkers
Osteoarthritis
Proteomics
Cartilage
Peroxiredoxin VI
Proteins
Chemotactic Factors
Articular Cartilage
Brain
Control Groups
Femoral Neck Fractures
Leukemia
Leukocytes
Western Blotting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Health, Toxicology and Mutagenesis

Cite this

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abstract = "Objective: We performed comprehensive proteomic analyses of articular cartilage by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and searched for candidate biomarkers for osteoarthritis (OA). Methods: Articular cartilage was collected from patients with OA or femoral neck fracture for the control group. Molecular variations were detected by the iTRAQ method, and quantitative analyses were performed by western blot. Results: Using the iTRAQ method, we identified 76 proteins with different expression levels in OA patients and the control group. Among these proteins, we selected LECT2 (leukocyte cell-derived chemotaxin-2), BAALC (brain and acute leukemia, cytoplasmic), and PRDX6 (peroxiredoxin-6), which had not been reported as biomarkers for OA. Conclusions: Use of these proteins in combination with conventional OA biomarkers may better reflect the grade and prognosis of OA.",
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ITRAQ-based proteomics reveals novel biomarkers of osteoarthritis. / Ikeda, Daiki; Ageta, Hiroshi; Tsuchida, Kunihiro; Yamada, Harumoto.

In: Biomarkers, Vol. 18, No. 7, 01.11.2013, p. 565-572.

Research output: Contribution to journalArticle

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AU - Ikeda, Daiki

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AU - Tsuchida, Kunihiro

AU - Yamada, Harumoto

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