Kinetic properties of Ash/Grb2-interacting GDP/GTP exchange protein

Hiroyuki Nakanishi, Satoshi Orita, Kozo Kaibuchi, Kenii Miura, Hiroaki Miki, Tadaomi Takenawa, Yoshimi Takai

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Ash/Grb2 is a protein having one SH2 domain flanked by two SH3 domains and is implicated to serve as an adaptor protein which links the EGF receptor to mammalian Sos (mSos), a GDP/GTP exchange protein for Ras. We isolated here several Ash-interacting proteins from bovine brain cytosol by use of glutathione-S-trasferase-Ash-linked agarose column chromatography. The Ash-interacting proteins stimulated the GDP/GTP exchange reaction of Ki-Ras and Ha-Ras but not that of other small GTP-binding proteins including at least Rap1, RhoA, Rac1, and Rab3A. The Ash-interacting proteins were much more active on the post-translationally lipid-modified form of Ki-Ras than on the unmodified form. At least one of them was identified as mSos by Western blot analysis using a specific anti-mSos antibody.

Original languageEnglish
Pages (from-to)1255-1261
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 14-02-1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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