Kinetics and collagenolytic role of eosinophils in chronic gastric ulcer in the rat

Naoki Ohmiya, Shinsuke Saga, Masaharu Ohbayashi, Ken Ichi Kozaki, Osamu Miyaishi, Miya Kobayashi, Shiro Kasuya, Tomiyasu Arisawa, Hidemi Goto, Tetsuo Hayakawa

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Abstract

An association between eosinophils and tissue damage has been observed in numerous disorders. However, few reports have addressed the role of infiltrating eosinophils in gastric ulcer healing. The aim of this study was to investigate the kinetics and role of eosinophils infiltrating experimental chronic gastric ulcers in the rat. We developed a monoclonal antibody against human matrix metalloproteinase 1 (MMP1) purified from conditioned culture medium of human skin fibroblasts. Acetic acid-induced gastric ulcers were resected from rats on days 1,3,5, 10, 20, 40, and 180 after the days of induction (day 0). Tissue specimens were immunostained with this antibody anti examined with an electron microscope. Few eosinophils were observed in the granulation tissue until day 20. By days 40 and 180, MMP1-positive eosinophils had increased in the granulation tissue of open ulcers. Azan staining revealed dispersed collagen fibers around infiltrating eosinophils. In contrast, scars demonstrated, few eosinophils in fibrous tissue on days 40 and 180. Eosinophils which express MMP1 infiltrate granulation tissue at the chronic stage of gastric ulceration. The results suggest that eosinophils may play a role in tissue remodeling and deterioration of ulceration.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalHistochemistry and Cell Biology
Volume108
Issue number1
DOIs
Publication statusPublished - 13-08-1997

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Stomach Ulcer
Eosinophils
Granulation Tissue
Matrix Metalloproteinase 1
Conditioned Culture Medium
Acetic Acid
Ulcer
Cicatrix
Anti-Idiotypic Antibodies
Stomach
Collagen
Fibroblasts
Monoclonal Antibodies
Electrons
Staining and Labeling
Skin

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Ohmiya, N., Saga, S., Ohbayashi, M., Kozaki, K. I., Miyaishi, O., Kobayashi, M., ... Hayakawa, T. (1997). Kinetics and collagenolytic role of eosinophils in chronic gastric ulcer in the rat. Histochemistry and Cell Biology, 108(1), 27-34. https://doi.org/10.1007/s004180050143
Ohmiya, Naoki ; Saga, Shinsuke ; Ohbayashi, Masaharu ; Kozaki, Ken Ichi ; Miyaishi, Osamu ; Kobayashi, Miya ; Kasuya, Shiro ; Arisawa, Tomiyasu ; Goto, Hidemi ; Hayakawa, Tetsuo. / Kinetics and collagenolytic role of eosinophils in chronic gastric ulcer in the rat. In: Histochemistry and Cell Biology. 1997 ; Vol. 108, No. 1. pp. 27-34.
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abstract = "An association between eosinophils and tissue damage has been observed in numerous disorders. However, few reports have addressed the role of infiltrating eosinophils in gastric ulcer healing. The aim of this study was to investigate the kinetics and role of eosinophils infiltrating experimental chronic gastric ulcers in the rat. We developed a monoclonal antibody against human matrix metalloproteinase 1 (MMP1) purified from conditioned culture medium of human skin fibroblasts. Acetic acid-induced gastric ulcers were resected from rats on days 1,3,5, 10, 20, 40, and 180 after the days of induction (day 0). Tissue specimens were immunostained with this antibody anti examined with an electron microscope. Few eosinophils were observed in the granulation tissue until day 20. By days 40 and 180, MMP1-positive eosinophils had increased in the granulation tissue of open ulcers. Azan staining revealed dispersed collagen fibers around infiltrating eosinophils. In contrast, scars demonstrated, few eosinophils in fibrous tissue on days 40 and 180. Eosinophils which express MMP1 infiltrate granulation tissue at the chronic stage of gastric ulceration. The results suggest that eosinophils may play a role in tissue remodeling and deterioration of ulceration.",
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Ohmiya, N, Saga, S, Ohbayashi, M, Kozaki, KI, Miyaishi, O, Kobayashi, M, Kasuya, S, Arisawa, T, Goto, H & Hayakawa, T 1997, 'Kinetics and collagenolytic role of eosinophils in chronic gastric ulcer in the rat', Histochemistry and Cell Biology, vol. 108, no. 1, pp. 27-34. https://doi.org/10.1007/s004180050143

Kinetics and collagenolytic role of eosinophils in chronic gastric ulcer in the rat. / Ohmiya, Naoki; Saga, Shinsuke; Ohbayashi, Masaharu; Kozaki, Ken Ichi; Miyaishi, Osamu; Kobayashi, Miya; Kasuya, Shiro; Arisawa, Tomiyasu; Goto, Hidemi; Hayakawa, Tetsuo.

In: Histochemistry and Cell Biology, Vol. 108, No. 1, 13.08.1997, p. 27-34.

Research output: Contribution to journalArticle

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AU - Ohmiya, Naoki

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AU - Miyaishi, Osamu

AU - Kobayashi, Miya

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AU - Arisawa, Tomiyasu

AU - Goto, Hidemi

AU - Hayakawa, Tetsuo

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Y1 - 1997/8/13

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