TY - JOUR
T1 - Kinetics and collagenolytic role of eosinophils in chronic gastric ulcer in the rat
AU - Ohmiya, Naoki
AU - Saga, Shinsuke
AU - Ohbayashi, Masaharu
AU - Kozaki, Ken Ichi
AU - Miyaishi, Osamu
AU - Kobayashi, Miya
AU - Kasuya, Shiro
AU - Arisawa, Tomiyasu
AU - Goto, Hidemi
AU - Hayakawa, Tetsuo
PY - 1997
Y1 - 1997
N2 - An association between eosinophils and tissue damage has been observed in numerous disorders. However, few reports have addressed the role of infiltrating eosinophils in gastric ulcer healing. The aim of this study was to investigate the kinetics and role of eosinophils infiltrating experimental chronic gastric ulcers in the rat. We developed a monoclonal antibody against human matrix metalloproteinase 1 (MMP1) purified from conditioned culture medium of human skin fibroblasts. Acetic acid-induced gastric ulcers were resected from rats on days 1,3,5, 10, 20, 40, and 180 after the days of induction (day 0). Tissue specimens were immunostained with this antibody anti examined with an electron microscope. Few eosinophils were observed in the granulation tissue until day 20. By days 40 and 180, MMP1-positive eosinophils had increased in the granulation tissue of open ulcers. Azan staining revealed dispersed collagen fibers around infiltrating eosinophils. In contrast, scars demonstrated, few eosinophils in fibrous tissue on days 40 and 180. Eosinophils which express MMP1 infiltrate granulation tissue at the chronic stage of gastric ulceration. The results suggest that eosinophils may play a role in tissue remodeling and deterioration of ulceration.
AB - An association between eosinophils and tissue damage has been observed in numerous disorders. However, few reports have addressed the role of infiltrating eosinophils in gastric ulcer healing. The aim of this study was to investigate the kinetics and role of eosinophils infiltrating experimental chronic gastric ulcers in the rat. We developed a monoclonal antibody against human matrix metalloproteinase 1 (MMP1) purified from conditioned culture medium of human skin fibroblasts. Acetic acid-induced gastric ulcers were resected from rats on days 1,3,5, 10, 20, 40, and 180 after the days of induction (day 0). Tissue specimens were immunostained with this antibody anti examined with an electron microscope. Few eosinophils were observed in the granulation tissue until day 20. By days 40 and 180, MMP1-positive eosinophils had increased in the granulation tissue of open ulcers. Azan staining revealed dispersed collagen fibers around infiltrating eosinophils. In contrast, scars demonstrated, few eosinophils in fibrous tissue on days 40 and 180. Eosinophils which express MMP1 infiltrate granulation tissue at the chronic stage of gastric ulceration. The results suggest that eosinophils may play a role in tissue remodeling and deterioration of ulceration.
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U2 - 10.1007/s004180050143
DO - 10.1007/s004180050143
M3 - Article
C2 - 9377222
AN - SCOPUS:8544239326
SN - 0948-6143
VL - 108
SP - 27
EP - 34
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 1
ER -