Kinetics of monocytoid B lymphocytes in localized inflammatory lesions induced by lipopolysaccharide in mice

Masahiko Fujino, Masaharu Ohbayashi, Naoki Omiya, Nobuo Kato, Junpei Asai, Masafumi Ito

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Immunohistochemistry was used to study the kinetics of B lymphocytes (B- lys) in the early stages of the localized inflammatory response induced in SMA mice by the subcutaneous injection of lipopolysaccharide (LPS). At the injection sites, medium-sized B-lys formed early inflammatory lesions with neutrophils and activated macrophages on days 1 and 2. The B-lys were morphologically similar to monocytes, but were not stained with Mac1 antibody. Remarkably the B-lys showed the phenotypes of B220+, IgM+, IgD (slight to negative), Ly-1- and CD23- by double immunohistochemical staining. The B-lys were also positive for alkaline phosphatase. Consequently the B-lys could be identified as monocytoid B-lys or marginal zone B-lys. Plasmacytic B-lys and plasma cells were first observed on days 3 and 4, but no lymphoid follicles were found at the injection sites. In the inguinal lymph nodes, the same B-lys responses were mainly induced in the paracortical lesions (T cell areas) preceding the formation of activated germinal centers (GC). These findings suggested that the B-lys, induced by injections of LPS, matured into plasma cells in the localized inflammatory lesions independent of GC, and that they were different from follicular B-lys.

Original languageEnglish
Pages (from-to)399-407
Number of pages9
JournalPathology International
Volume46
Issue number6
DOIs
Publication statusPublished - 01-01-1996
Externally publishedYes

Fingerprint

Lipopolysaccharides
B-Lymphocytes
Germinal Center
Plasma Cells
Injections
Immunoglobulin D
Groin
Subcutaneous Injections
Alkaline Phosphatase
Immunoglobulin M
Monocytes
Neutrophils
Lymph Nodes
Immunohistochemistry
Macrophages
Staining and Labeling
T-Lymphocytes
Phenotype
Antibodies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Fujino, Masahiko ; Ohbayashi, Masaharu ; Omiya, Naoki ; Kato, Nobuo ; Asai, Junpei ; Ito, Masafumi. / Kinetics of monocytoid B lymphocytes in localized inflammatory lesions induced by lipopolysaccharide in mice. In: Pathology International. 1996 ; Vol. 46, No. 6. pp. 399-407.
@article{c47a5f3bb2104f62a085b5488d4ffa4d,
title = "Kinetics of monocytoid B lymphocytes in localized inflammatory lesions induced by lipopolysaccharide in mice",
abstract = "Immunohistochemistry was used to study the kinetics of B lymphocytes (B- lys) in the early stages of the localized inflammatory response induced in SMA mice by the subcutaneous injection of lipopolysaccharide (LPS). At the injection sites, medium-sized B-lys formed early inflammatory lesions with neutrophils and activated macrophages on days 1 and 2. The B-lys were morphologically similar to monocytes, but were not stained with Mac1 antibody. Remarkably the B-lys showed the phenotypes of B220+, IgM+, IgD (slight to negative), Ly-1- and CD23- by double immunohistochemical staining. The B-lys were also positive for alkaline phosphatase. Consequently the B-lys could be identified as monocytoid B-lys or marginal zone B-lys. Plasmacytic B-lys and plasma cells were first observed on days 3 and 4, but no lymphoid follicles were found at the injection sites. In the inguinal lymph nodes, the same B-lys responses were mainly induced in the paracortical lesions (T cell areas) preceding the formation of activated germinal centers (GC). These findings suggested that the B-lys, induced by injections of LPS, matured into plasma cells in the localized inflammatory lesions independent of GC, and that they were different from follicular B-lys.",
author = "Masahiko Fujino and Masaharu Ohbayashi and Naoki Omiya and Nobuo Kato and Junpei Asai and Masafumi Ito",
year = "1996",
month = "1",
day = "1",
doi = "10.1111/j.1440-1827.1996.tb03630.x",
language = "English",
volume = "46",
pages = "399--407",
journal = "Pathology International",
issn = "1320-5463",
publisher = "Wiley-Blackwell",
number = "6",

}

Kinetics of monocytoid B lymphocytes in localized inflammatory lesions induced by lipopolysaccharide in mice. / Fujino, Masahiko; Ohbayashi, Masaharu; Omiya, Naoki; Kato, Nobuo; Asai, Junpei; Ito, Masafumi.

In: Pathology International, Vol. 46, No. 6, 01.01.1996, p. 399-407.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Kinetics of monocytoid B lymphocytes in localized inflammatory lesions induced by lipopolysaccharide in mice

AU - Fujino, Masahiko

AU - Ohbayashi, Masaharu

AU - Omiya, Naoki

AU - Kato, Nobuo

AU - Asai, Junpei

AU - Ito, Masafumi

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Immunohistochemistry was used to study the kinetics of B lymphocytes (B- lys) in the early stages of the localized inflammatory response induced in SMA mice by the subcutaneous injection of lipopolysaccharide (LPS). At the injection sites, medium-sized B-lys formed early inflammatory lesions with neutrophils and activated macrophages on days 1 and 2. The B-lys were morphologically similar to monocytes, but were not stained with Mac1 antibody. Remarkably the B-lys showed the phenotypes of B220+, IgM+, IgD (slight to negative), Ly-1- and CD23- by double immunohistochemical staining. The B-lys were also positive for alkaline phosphatase. Consequently the B-lys could be identified as monocytoid B-lys or marginal zone B-lys. Plasmacytic B-lys and plasma cells were first observed on days 3 and 4, but no lymphoid follicles were found at the injection sites. In the inguinal lymph nodes, the same B-lys responses were mainly induced in the paracortical lesions (T cell areas) preceding the formation of activated germinal centers (GC). These findings suggested that the B-lys, induced by injections of LPS, matured into plasma cells in the localized inflammatory lesions independent of GC, and that they were different from follicular B-lys.

AB - Immunohistochemistry was used to study the kinetics of B lymphocytes (B- lys) in the early stages of the localized inflammatory response induced in SMA mice by the subcutaneous injection of lipopolysaccharide (LPS). At the injection sites, medium-sized B-lys formed early inflammatory lesions with neutrophils and activated macrophages on days 1 and 2. The B-lys were morphologically similar to monocytes, but were not stained with Mac1 antibody. Remarkably the B-lys showed the phenotypes of B220+, IgM+, IgD (slight to negative), Ly-1- and CD23- by double immunohistochemical staining. The B-lys were also positive for alkaline phosphatase. Consequently the B-lys could be identified as monocytoid B-lys or marginal zone B-lys. Plasmacytic B-lys and plasma cells were first observed on days 3 and 4, but no lymphoid follicles were found at the injection sites. In the inguinal lymph nodes, the same B-lys responses were mainly induced in the paracortical lesions (T cell areas) preceding the formation of activated germinal centers (GC). These findings suggested that the B-lys, induced by injections of LPS, matured into plasma cells in the localized inflammatory lesions independent of GC, and that they were different from follicular B-lys.

UR - http://www.scopus.com/inward/record.url?scp=0029786658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029786658&partnerID=8YFLogxK

U2 - 10.1111/j.1440-1827.1996.tb03630.x

DO - 10.1111/j.1440-1827.1996.tb03630.x

M3 - Article

VL - 46

SP - 399

EP - 407

JO - Pathology International

JF - Pathology International

SN - 1320-5463

IS - 6

ER -