TY - JOUR
T1 - Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres
AU - Manabe, Takayuki
AU - Tatsumi, Kouko
AU - Inoue, Masahide
AU - Matsuyoshi, Hiroko
AU - Makinodan, Manabu
AU - Yamauchi, Takahira
AU - Makinodan, Eri
AU - Yokoyama, Shohei
AU - Sakumura, Ryoko
AU - Okuda, Hiroaki
AU - Wanaka, Akio
PY - 2008/4
Y1 - 2008/4
N2 - L3/Lhx8, a member of the Lim-homeobox gene family, is selectively and specifically expressed in the murine embryonic medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-deficient mice specifically lack cholinergic neurons in the basal forebrain. In this manuscript, we report the in vitro effects of reduced L3/Lhx8 gene expression on cholinergic differentiation in murine embryonic stem (ES) cell-derived spheres without dissociation. The knockdown of L3/Lhx8 gene expression dramatically decreased the cholinergic phenotype of spheres without altering other known phenotypes (TuJ1, GABA and GFAP). These results strongly suggest that L3/Lhx8 is a key factor for cholinergic differentiation of murine ES cell-derived spheres and is involved in basal forebrain development.
AB - L3/Lhx8, a member of the Lim-homeobox gene family, is selectively and specifically expressed in the murine embryonic medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-deficient mice specifically lack cholinergic neurons in the basal forebrain. In this manuscript, we report the in vitro effects of reduced L3/Lhx8 gene expression on cholinergic differentiation in murine embryonic stem (ES) cell-derived spheres without dissociation. The knockdown of L3/Lhx8 gene expression dramatically decreased the cholinergic phenotype of spheres without altering other known phenotypes (TuJ1, GABA and GFAP). These results strongly suggest that L3/Lhx8 is a key factor for cholinergic differentiation of murine ES cell-derived spheres and is involved in basal forebrain development.
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U2 - 10.1016/j.ijdevneu.2007.11.004
DO - 10.1016/j.ijdevneu.2007.11.004
M3 - Article
C2 - 18248940
AN - SCOPUS:39149120579
SN - 0736-5748
VL - 26
SP - 249
EP - 252
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
IS - 2
ER -