Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres

Takayuki Manabe, Kouko Tatsumi, Masahide Inoue, Hiroko Matsuyoshi, Manabu Makinodan, Takahira Yamauchi, Eri Makinodan, Shohei Yokoyama, Ryoko Sakumura, Hiroaki Okuda, Akio Wanaka

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

L3/Lhx8, a member of the Lim-homeobox gene family, is selectively and specifically expressed in the murine embryonic medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-deficient mice specifically lack cholinergic neurons in the basal forebrain. In this manuscript, we report the in vitro effects of reduced L3/Lhx8 gene expression on cholinergic differentiation in murine embryonic stem (ES) cell-derived spheres without dissociation. The knockdown of L3/Lhx8 gene expression dramatically decreased the cholinergic phenotype of spheres without altering other known phenotypes (TuJ1, GABA and GFAP). These results strongly suggest that L3/Lhx8 is a key factor for cholinergic differentiation of murine ES cell-derived spheres and is involved in basal forebrain development.

Original languageEnglish
Pages (from-to)249-252
Number of pages4
JournalInternational Journal of Developmental Neuroscience
Volume26
Issue number2
DOIs
Publication statusPublished - 04-2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience
  • Developmental Biology

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