Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres

Takayuki Manabe; Kouko Tatsumi; Masahide Inoue; Hiroko Matsuyoshi; Manabu Makinodan; Takahira Yamauchi; Eri Makinodan; Shohei Yokoyama; Ryoko Sakumura; Hiroaki Okuda; Akio Wanaka

doi:10.1016/j.ijdevneu.2007.11.004

Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres

Takayuki Manabe
, Kouko Tatsumi
, Masahide Inoue
, Hiroko Matsuyoshi
, Manabu Makinodan
, Takahira Yamauchi
, Eri Makinodan
, Shohei Yokoyama
, Ryoko Sakumura
, Hiroaki Okuda
, Akio Wanaka

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

L3/Lhx8, a member of the Lim-homeobox gene family, is selectively and specifically expressed in the murine embryonic medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-deficient mice specifically lack cholinergic neurons in the basal forebrain. In this manuscript, we report the in vitro effects of reduced L3/Lhx8 gene expression on cholinergic differentiation in murine embryonic stem (ES) cell-derived spheres without dissociation. The knockdown of L3/Lhx8 gene expression dramatically decreased the cholinergic phenotype of spheres without altering other known phenotypes (TuJ1, GABA and GFAP). These results strongly suggest that L3/Lhx8 is a key factor for cholinergic differentiation of murine ES cell-derived spheres and is involved in basal forebrain development.

Original language	English
Pages (from-to)	249-252
Number of pages	4
Journal	International Journal of Developmental Neuroscience
Volume	26
Issue number	2
DOIs	https://doi.org/10.1016/j.ijdevneu.2007.11.004
Publication status	Published - 04-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Developmental Neuroscience
Developmental Biology

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10.1016/j.ijdevneu.2007.11.004

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Manabe, T., Tatsumi, K., Inoue, M., Matsuyoshi, H., Makinodan, M., Yamauchi, T., Makinodan, E., Yokoyama, S., Sakumura, R., Okuda, H., & Wanaka, A. (2008). Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres. International Journal of Developmental Neuroscience, 26(2), 249-252. https://doi.org/10.1016/j.ijdevneu.2007.11.004

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title = "Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres",

abstract = "L3/Lhx8, a member of the Lim-homeobox gene family, is selectively and specifically expressed in the murine embryonic medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-deficient mice specifically lack cholinergic neurons in the basal forebrain. In this manuscript, we report the in vitro effects of reduced L3/Lhx8 gene expression on cholinergic differentiation in murine embryonic stem (ES) cell-derived spheres without dissociation. The knockdown of L3/Lhx8 gene expression dramatically decreased the cholinergic phenotype of spheres without altering other known phenotypes (TuJ1, GABA and GFAP). These results strongly suggest that L3/Lhx8 is a key factor for cholinergic differentiation of murine ES cell-derived spheres and is involved in basal forebrain development.",

author = "Takayuki Manabe and Kouko Tatsumi and Masahide Inoue and Hiroko Matsuyoshi and Manabu Makinodan and Takahira Yamauchi and Eri Makinodan and Shohei Yokoyama and Ryoko Sakumura and Hiroaki Okuda and Akio Wanaka",

year = "2008",

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doi = "10.1016/j.ijdevneu.2007.11.004",

language = "English",

volume = "26",

pages = "249--252",

journal = "International Journal of Developmental Neuroscience",

issn = "0736-5748",

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}

Manabe, T, Tatsumi, K, Inoue, M, Matsuyoshi, H, Makinodan, M, Yamauchi, T, Makinodan, E, Yokoyama, S, Sakumura, R, Okuda, H & Wanaka, A 2008, 'Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres', International Journal of Developmental Neuroscience, vol. 26, no. 2, pp. 249-252. https://doi.org/10.1016/j.ijdevneu.2007.11.004

TY - JOUR

T1 - Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres

AU - Manabe, Takayuki

AU - Tatsumi, Kouko

AU - Inoue, Masahide

AU - Matsuyoshi, Hiroko

AU - Makinodan, Manabu

AU - Yamauchi, Takahira

AU - Makinodan, Eri

AU - Yokoyama, Shohei

AU - Sakumura, Ryoko

AU - Okuda, Hiroaki

AU - Wanaka, Akio

PY - 2008/4

Y1 - 2008/4

N2 - L3/Lhx8, a member of the Lim-homeobox gene family, is selectively and specifically expressed in the murine embryonic medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-deficient mice specifically lack cholinergic neurons in the basal forebrain. In this manuscript, we report the in vitro effects of reduced L3/Lhx8 gene expression on cholinergic differentiation in murine embryonic stem (ES) cell-derived spheres without dissociation. The knockdown of L3/Lhx8 gene expression dramatically decreased the cholinergic phenotype of spheres without altering other known phenotypes (TuJ1, GABA and GFAP). These results strongly suggest that L3/Lhx8 is a key factor for cholinergic differentiation of murine ES cell-derived spheres and is involved in basal forebrain development.

AB - L3/Lhx8, a member of the Lim-homeobox gene family, is selectively and specifically expressed in the murine embryonic medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-deficient mice specifically lack cholinergic neurons in the basal forebrain. In this manuscript, we report the in vitro effects of reduced L3/Lhx8 gene expression on cholinergic differentiation in murine embryonic stem (ES) cell-derived spheres without dissociation. The knockdown of L3/Lhx8 gene expression dramatically decreased the cholinergic phenotype of spheres without altering other known phenotypes (TuJ1, GABA and GFAP). These results strongly suggest that L3/Lhx8 is a key factor for cholinergic differentiation of murine ES cell-derived spheres and is involved in basal forebrain development.

UR - https://www.scopus.com/pages/publications/39149120579

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DO - 10.1016/j.ijdevneu.2007.11.004

M3 - Article

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AN - SCOPUS:39149120579

SN - 0736-5748

VL - 26

SP - 249

EP - 252

JO - International Journal of Developmental Neuroscience

JF - International Journal of Developmental Neuroscience

IS - 2

ER -

Knockdown of the L3/Lhx8 gene suppresses cholinergic differentiation of murine embryonic stem cell-derived spheres

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