L-tryptophan-kynurenine pathway metabolite 3-hydroxyanthranilic acid induces apoptosis in macrophage-derived cells under pathophysiological conditions

Toshiko Morita, Kuniaki Saito, Masao Takemura, Naoya Maekawa, Suwako Fujigaki, Hidehiko Fujii, Hisayasu Wada, Shoji Takeuchi, Akio Noma, Mitsuru Seishima

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Accumulation of L-kynurenine and 3-hydroxyanthranilic acid (3HAA) occurs in the monocyte-derived cells following immune stimulation, and may derive from L-tryptophan following induction of indoleamine-2,3-dioxygenase. In the present study, we evaluate the possibility that 3HAA acts as an endogenous inducer of monocyte/macrophage apoptosis. Supplementation with 200 μM of 3HAA, but not other L-tryptophan metabolites tested, significantly increased the number of apoptotic cells in both THP-1 and U937 cells. Catalase, superoxide dismutase and manganese ions markedly enhanced apoptosis in the presence of 3HAA in these cells. The present results suggest that 3HAA induces the macrophage/monocyte apoptosis under certain conditions, which may be relevant to pathophysiology of inflammatory conditions.

Original languageEnglish
Pages (from-to)559-563
Number of pages5
JournalAdvances in Experimental Medicine and Biology
Volume467
Publication statusPublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology

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