L-tryptophan-kynurenine pathway metabolite 3-hydroxyanthranilic acid induces apoptosis in macrophage-derived cells under pathophysiological conditions

Toshiko Morita, Kuniaki Saito, Masao Takemura, Naoya Maekawa, Suwako Fujigaki, Hidehiko Fujii, Hisayasu Wada, Shoji Takeuchi, Akio Noma, Mitsuru Seishima

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Accumulation of L-kynurenine and 3-hydroxyanthranilic acid (3HAA) occurs in the monocyte-derived cells following immune stimulation, and may derive from L-tryptophan following induction of indoleamine-2,3-dioxygenase. In the present study, we evaluate the possibility that 3HAA acts as an endogenous inducer of monocyte/macrophage apoptosis. Supplementation with 200 μM of 3HAA, but not other L-tryptophan metabolites tested, significantly increased the number of apoptotic cells in both THP-1 and U937 cells. Catalase, superoxide dismutase and manganese ions markedly enhanced apoptosis in the presence of 3HAA in these cells. The present results suggest that 3HAA induces the macrophage/monocyte apoptosis under certain conditions, which may be relevant to pathophysiology of inflammatory conditions.

Original languageEnglish
Pages (from-to)559-563
Number of pages5
JournalAdvances in Experimental Medicine and Biology
Volume467
Publication statusPublished - 01-01-2000
Externally publishedYes

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3-Hydroxyanthranilic Acid
Kynurenine
Macrophages
Metabolites
Tryptophan
Apoptosis
Monocytes
Indoleamine-Pyrrole 2,3,-Dioxygenase
U937 Cells
Catalase
Superoxide Dismutase
Cell Count
Cells
Ions

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Morita, Toshiko ; Saito, Kuniaki ; Takemura, Masao ; Maekawa, Naoya ; Fujigaki, Suwako ; Fujii, Hidehiko ; Wada, Hisayasu ; Takeuchi, Shoji ; Noma, Akio ; Seishima, Mitsuru. / L-tryptophan-kynurenine pathway metabolite 3-hydroxyanthranilic acid induces apoptosis in macrophage-derived cells under pathophysiological conditions. In: Advances in Experimental Medicine and Biology. 2000 ; Vol. 467. pp. 559-563.
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Morita, T, Saito, K, Takemura, M, Maekawa, N, Fujigaki, S, Fujii, H, Wada, H, Takeuchi, S, Noma, A & Seishima, M 2000, 'L-tryptophan-kynurenine pathway metabolite 3-hydroxyanthranilic acid induces apoptosis in macrophage-derived cells under pathophysiological conditions', Advances in Experimental Medicine and Biology, vol. 467, pp. 559-563.

L-tryptophan-kynurenine pathway metabolite 3-hydroxyanthranilic acid induces apoptosis in macrophage-derived cells under pathophysiological conditions. / Morita, Toshiko; Saito, Kuniaki; Takemura, Masao; Maekawa, Naoya; Fujigaki, Suwako; Fujii, Hidehiko; Wada, Hisayasu; Takeuchi, Shoji; Noma, Akio; Seishima, Mitsuru.

In: Advances in Experimental Medicine and Biology, Vol. 467, 01.01.2000, p. 559-563.

Research output: Contribution to journalArticle

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AU - Morita, Toshiko

AU - Saito, Kuniaki

AU - Takemura, Masao

AU - Maekawa, Naoya

AU - Fujigaki, Suwako

AU - Fujii, Hidehiko

AU - Wada, Hisayasu

AU - Takeuchi, Shoji

AU - Noma, Akio

AU - Seishima, Mitsuru

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AB - Accumulation of L-kynurenine and 3-hydroxyanthranilic acid (3HAA) occurs in the monocyte-derived cells following immune stimulation, and may derive from L-tryptophan following induction of indoleamine-2,3-dioxygenase. In the present study, we evaluate the possibility that 3HAA acts as an endogenous inducer of monocyte/macrophage apoptosis. Supplementation with 200 μM of 3HAA, but not other L-tryptophan metabolites tested, significantly increased the number of apoptotic cells in both THP-1 and U937 cells. Catalase, superoxide dismutase and manganese ions markedly enhanced apoptosis in the presence of 3HAA in these cells. The present results suggest that 3HAA induces the macrophage/monocyte apoptosis under certain conditions, which may be relevant to pathophysiology of inflammatory conditions.

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