TY - JOUR
T1 - Lack of integrated TT virus (TTV) genomes into cellular DNA in infected human hematopoietic cells
AU - Tanaka, Yasuhito
AU - Mizokami, Masashi
AU - Orito, Etsuro
AU - Ohno, Tomoyoshi
AU - Nakano, Tatsunori
AU - Kato, Takanobu
AU - Iida, Shinsuke
AU - Ueda, Ryuzo
N1 - Funding Information:
The authors would like to thank Dr. Hijikata and Dr. Mishiro, Department of Medical Science, Toshiba General Hospital, Tokyo, for providing primers of TTV, and C. Fukuyama for technical assistance. This work was partly supported by Grants-in Aid from the Ministry of Education and Culture and the Ministry of Health and Welfare.
PY - 2000
Y1 - 2000
N2 - TT virus (TTV) isolated from the serum of a patient with posttransfusion hepatitis has been characterized as a member of the Circoviridae, a family of small DNA viruses with single-stranded circular genomes. TTV appeared to infect not only the serum and liver, but also the peripheral blood mononuclear cells (PBMC). We investigated the prevalence of TTV DNA in human hematopoietic cells, based on 84 mononuclear cell samples obtained from the bone marrow or lymph nodes of patients with hematopoietic malignancies including leukemia, malignant lymphoma and aplastic anemia. Forty-nine (58.3%) out of the 84 samples were positive for TTV DNA with polymerase chain reaction analysis, which was almost similar to the frequency found in the patients' serum. Southern blot analyses using a 3.2-kb fragment derived from the TTV DNA, however, showed no evidence supporting the fact that the TTV genomes are integrated into the human hematopoietic cell genomes, thus suggesting their existence as episomal forms.
AB - TT virus (TTV) isolated from the serum of a patient with posttransfusion hepatitis has been characterized as a member of the Circoviridae, a family of small DNA viruses with single-stranded circular genomes. TTV appeared to infect not only the serum and liver, but also the peripheral blood mononuclear cells (PBMC). We investigated the prevalence of TTV DNA in human hematopoietic cells, based on 84 mononuclear cell samples obtained from the bone marrow or lymph nodes of patients with hematopoietic malignancies including leukemia, malignant lymphoma and aplastic anemia. Forty-nine (58.3%) out of the 84 samples were positive for TTV DNA with polymerase chain reaction analysis, which was almost similar to the frequency found in the patients' serum. Southern blot analyses using a 3.2-kb fragment derived from the TTV DNA, however, showed no evidence supporting the fact that the TTV genomes are integrated into the human hematopoietic cell genomes, thus suggesting their existence as episomal forms.
UR - http://www.scopus.com/inward/record.url?scp=0034102832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034102832&partnerID=8YFLogxK
U2 - 10.3109/10428190009087033
DO - 10.3109/10428190009087033
M3 - Article
C2 - 10830749
AN - SCOPUS:0034102832
SN - 1042-8194
VL - 38
SP - 411
EP - 417
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 3-4
ER -
