Lack of interleukin-1ß decreases the severity of atherosclerosis in apoE-deficient mice

Hirokazu Kirii, Tamikazu Niwa, Yasuhiro Yamada, Hisayasu Wada, Kuniaki Saito, Yoichiro Iwakura, Masahide Asano, Hisataka Moriwaki, Mitsuru Seishima

Research output: Contribution to journalArticle

436 Citations (Scopus)

Abstract

Objective - Atherosclerosis is considered to be a chronic inflammatory disease and many cytokines participate in the development of atherosclerosis, We focused on the role of interleukin-1β (IL-1β, one of the proinflammatory cytokines secreted by monocytes/macrophages, in the progression of atherosclerosis. Methods and Results - We generated mice lacking both apoE and IL-1β. The sizes of atherosclerotic lesions at the aortic sinus in apoE-/-/IL-1β-/- mice at 12 and 24 weeks of age showed a significant decrease of approximately 30% compared with apoE-/-/IL-1β+/+ mice, and the percentage of the atherosclerotic area to total area of apoE-/-/IL-1β-/- at 24 weeks of age also showed a significant decrease of about 30% compared with apoE-/-/IL-1β+/+. The mRNA levels of vascular cell adhesion molecule (VCAM)-1 and monocyte chemotactic protein-1 in the apoE-/-/ IL-1β-/- aorta were significantly reduced compared with the apoE-/-/IL-1β+/+. Furthermore, VCAM-1 was also reduced at the protein level in apoE-/-/IL-1β-/- aorta compared with apoE-/-/IL-1β+/+. Conclusions - The lack of IL-1β decreases the severity of atherosclerosis in apoE deficient mice, possibly through increased expressions of VCAM-1 and monocyte chemotactic protein-1 in the aorta.

Original languageEnglish
Pages (from-to)656-660
Number of pages5
JournalArteriosclerosis, thrombosis, and vascular biology
Volume23
Issue number4
DOIs
Publication statusPublished - 01-04-2003

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Apolipoproteins E
Interleukin-1
Atherosclerosis
Vascular Cell Adhesion Molecule-1
Aorta
Chemokine CCL2
Cytokines
Sinus of Valsalva
Monocytes
Chronic Disease
Macrophages

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Kirii, Hirokazu ; Niwa, Tamikazu ; Yamada, Yasuhiro ; Wada, Hisayasu ; Saito, Kuniaki ; Iwakura, Yoichiro ; Asano, Masahide ; Moriwaki, Hisataka ; Seishima, Mitsuru. / Lack of interleukin-1ß decreases the severity of atherosclerosis in apoE-deficient mice. In: Arteriosclerosis, thrombosis, and vascular biology. 2003 ; Vol. 23, No. 4. pp. 656-660.
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Lack of interleukin-1ß decreases the severity of atherosclerosis in apoE-deficient mice. / Kirii, Hirokazu; Niwa, Tamikazu; Yamada, Yasuhiro; Wada, Hisayasu; Saito, Kuniaki; Iwakura, Yoichiro; Asano, Masahide; Moriwaki, Hisataka; Seishima, Mitsuru.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 23, No. 4, 01.04.2003, p. 656-660.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Lack of interleukin-1ß decreases the severity of atherosclerosis in apoE-deficient mice

AU - Kirii, Hirokazu

AU - Niwa, Tamikazu

AU - Yamada, Yasuhiro

AU - Wada, Hisayasu

AU - Saito, Kuniaki

AU - Iwakura, Yoichiro

AU - Asano, Masahide

AU - Moriwaki, Hisataka

AU - Seishima, Mitsuru

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Objective - Atherosclerosis is considered to be a chronic inflammatory disease and many cytokines participate in the development of atherosclerosis, We focused on the role of interleukin-1β (IL-1β, one of the proinflammatory cytokines secreted by monocytes/macrophages, in the progression of atherosclerosis. Methods and Results - We generated mice lacking both apoE and IL-1β. The sizes of atherosclerotic lesions at the aortic sinus in apoE-/-/IL-1β-/- mice at 12 and 24 weeks of age showed a significant decrease of approximately 30% compared with apoE-/-/IL-1β+/+ mice, and the percentage of the atherosclerotic area to total area of apoE-/-/IL-1β-/- at 24 weeks of age also showed a significant decrease of about 30% compared with apoE-/-/IL-1β+/+. The mRNA levels of vascular cell adhesion molecule (VCAM)-1 and monocyte chemotactic protein-1 in the apoE-/-/ IL-1β-/- aorta were significantly reduced compared with the apoE-/-/IL-1β+/+. Furthermore, VCAM-1 was also reduced at the protein level in apoE-/-/IL-1β-/- aorta compared with apoE-/-/IL-1β+/+. Conclusions - The lack of IL-1β decreases the severity of atherosclerosis in apoE deficient mice, possibly through increased expressions of VCAM-1 and monocyte chemotactic protein-1 in the aorta.

AB - Objective - Atherosclerosis is considered to be a chronic inflammatory disease and many cytokines participate in the development of atherosclerosis, We focused on the role of interleukin-1β (IL-1β, one of the proinflammatory cytokines secreted by monocytes/macrophages, in the progression of atherosclerosis. Methods and Results - We generated mice lacking both apoE and IL-1β. The sizes of atherosclerotic lesions at the aortic sinus in apoE-/-/IL-1β-/- mice at 12 and 24 weeks of age showed a significant decrease of approximately 30% compared with apoE-/-/IL-1β+/+ mice, and the percentage of the atherosclerotic area to total area of apoE-/-/IL-1β-/- at 24 weeks of age also showed a significant decrease of about 30% compared with apoE-/-/IL-1β+/+. The mRNA levels of vascular cell adhesion molecule (VCAM)-1 and monocyte chemotactic protein-1 in the apoE-/-/ IL-1β-/- aorta were significantly reduced compared with the apoE-/-/IL-1β+/+. Furthermore, VCAM-1 was also reduced at the protein level in apoE-/-/IL-1β-/- aorta compared with apoE-/-/IL-1β+/+. Conclusions - The lack of IL-1β decreases the severity of atherosclerosis in apoE deficient mice, possibly through increased expressions of VCAM-1 and monocyte chemotactic protein-1 in the aorta.

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