Abstract
In order to study the pathogenesis of HHV-6 infection in central nervous system disorders, U251 cell line was infected with freshly isolated variant B HHV-6. Although IEA/ex 3 antigen (immediate early protein) was detected in infected cell nuclei, neither the presence of OHV-3 antigen (late antigen) nor production of infectious virus was demonstrated. These results indicate that abortive infection was established in the cells. After viral infection, the viral genome copy in the infected cells gradually decreased and became stable after 6 cell passages. At that point, HHV-6 gene expression was restricted to only 2 immediate early genes (U90 and U94). However, 12-O-tetra-decanoyl (TPA) treatment induced transcription of other genes (U31 and U39) by the 10th cell passage, indicating HHV-6 reactivation. Moreover, production of two proinflamatory cytokines (IL-6 and IL-1β) was up-regulated by the presence of the HHV-6 genome and TPA-induced activation of the viral transcripts.
| Original language | English |
|---|---|
| Pages (from-to) | 497-505 |
| Number of pages | 9 |
| Journal | Journal of Medical Virology |
| Volume | 66 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 19-03-2002 |
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All Science Journal Classification (ASJC) codes
- Virology
- Infectious Diseases
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Latent infection of human herpesvirus 6 in astrocytoma cell line and alteration of cytokine synthesis. / Yoshikawa, Tetsushi; Asano, Yoshizo; Akimoto, Shiho; Ozaki, Takao; Iwasaki, Takuya; Kurata, Takeshi; Goshima, Fumi; Nishiyama, Yukihiro.
In: Journal of Medical Virology, Vol. 66, No. 4, 19.03.2002, p. 497-505.Research output: Contribution to journal › Article
TY - JOUR
T1 - Latent infection of human herpesvirus 6 in astrocytoma cell line and alteration of cytokine synthesis
AU - Yoshikawa, Tetsushi
AU - Asano, Yoshizo
AU - Akimoto, Shiho
AU - Ozaki, Takao
AU - Iwasaki, Takuya
AU - Kurata, Takeshi
AU - Goshima, Fumi
AU - Nishiyama, Yukihiro
PY - 2002/3/19
Y1 - 2002/3/19
N2 - In order to study the pathogenesis of HHV-6 infection in central nervous system disorders, U251 cell line was infected with freshly isolated variant B HHV-6. Although IEA/ex 3 antigen (immediate early protein) was detected in infected cell nuclei, neither the presence of OHV-3 antigen (late antigen) nor production of infectious virus was demonstrated. These results indicate that abortive infection was established in the cells. After viral infection, the viral genome copy in the infected cells gradually decreased and became stable after 6 cell passages. At that point, HHV-6 gene expression was restricted to only 2 immediate early genes (U90 and U94). However, 12-O-tetra-decanoyl (TPA) treatment induced transcription of other genes (U31 and U39) by the 10th cell passage, indicating HHV-6 reactivation. Moreover, production of two proinflamatory cytokines (IL-6 and IL-1β) was up-regulated by the presence of the HHV-6 genome and TPA-induced activation of the viral transcripts.
AB - In order to study the pathogenesis of HHV-6 infection in central nervous system disorders, U251 cell line was infected with freshly isolated variant B HHV-6. Although IEA/ex 3 antigen (immediate early protein) was detected in infected cell nuclei, neither the presence of OHV-3 antigen (late antigen) nor production of infectious virus was demonstrated. These results indicate that abortive infection was established in the cells. After viral infection, the viral genome copy in the infected cells gradually decreased and became stable after 6 cell passages. At that point, HHV-6 gene expression was restricted to only 2 immediate early genes (U90 and U94). However, 12-O-tetra-decanoyl (TPA) treatment induced transcription of other genes (U31 and U39) by the 10th cell passage, indicating HHV-6 reactivation. Moreover, production of two proinflamatory cytokines (IL-6 and IL-1β) was up-regulated by the presence of the HHV-6 genome and TPA-induced activation of the viral transcripts.
UR - http://www.scopus.com/inward/record.url?scp=0036198471&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036198471&partnerID=8YFLogxK
U2 - 10.1002/jmv.2172
DO - 10.1002/jmv.2172
M3 - Article
C2 - 11857528
AN - SCOPUS:0036198471
VL - 66
SP - 497
EP - 505
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 4
ER -