Latent infection of human herpesvirus 7 in CD4+ T lymphocytes

Fumi Miyake, Tetsushi Yoshikawa, He Sun, Akira Kakimi, Masahiro Ohashi, Shiho Akimoto, Yukihiro Nishiyama, Yoshizo Asano

Research output: Contribution to journalArticle

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Abstract

To determine the cell populations in peripheral blood that are infected latently with human herpesvirus 7 (HHV-7), the real-time polymerase chain reaction (PCR) was used to determine the quantities of viral DNA in adherent and non-adherent cells from 71 healthy volunteers. Real-time PCR, which detected the U31 gene of HHV-7, was developed to measure viral load. The majority of non-adherent cells (14/16; 87.5%) contained HHV-7 DNA, while most of the adherent cells did not (1/16; 6.3%). HHV-7 viral load in non-adherent cells was significantly higher than that in adherent cells (P<0.0001). Then, HHV-7 DNA load was compared between the CD4-positive and -negative cell fractions derived from the non-adherent cells of 26 healthy adults. As in the previous experiment, only 2 (7.7%) of the 26 adherent cell specimens contained small amounts of HHV-7 DNA (27.7 copies/1 × 106 cells and 208.7 copies/1 × 106 cells). In contrast, 88.5% of CD4+ T cell samples (23/26 specimens) were positive for HHV-7 DNA, ranging from 0.4 to 3,542.8 copies/1 × 106 cells. Viral DNA was detected in only 3 (11.5%) of the 26 CD4- T cell specimens, with 8.4, 63.5, and 74.1 copies/1 × 106 cells. HHV-7-positive DNA loads were significantly higher in the CD4+ T cells than those observed in the CD4- T cells (P=0.0005). The relationship between HHV-7 viral loads in non-adherent cells and those in saliva was investigated. Comparison of HHV-7 DNA load between blood CD4+ T cells and saliva revealed that the HHV-7 DNA load in saliva correlated with that present in CD4+ T cells (r=0.415; P=0.0174).

Original languageEnglish
Pages (from-to)112-116
Number of pages5
JournalJournal of Medical Virology
Volume78
Issue number1
DOIs
Publication statusPublished - 01-01-2006

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Human Herpesvirus 7
T-Lymphocytes
Infection
DNA
Viral Load
Saliva
Viral DNA
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Miyake, F., Yoshikawa, T., Sun, H., Kakimi, A., Ohashi, M., Akimoto, S., ... Asano, Y. (2006). Latent infection of human herpesvirus 7 in CD4+ T lymphocytes. Journal of Medical Virology, 78(1), 112-116. https://doi.org/10.1002/jmv.20511
Miyake, Fumi ; Yoshikawa, Tetsushi ; Sun, He ; Kakimi, Akira ; Ohashi, Masahiro ; Akimoto, Shiho ; Nishiyama, Yukihiro ; Asano, Yoshizo. / Latent infection of human herpesvirus 7 in CD4+ T lymphocytes. In: Journal of Medical Virology. 2006 ; Vol. 78, No. 1. pp. 112-116.
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Miyake, F, Yoshikawa, T, Sun, H, Kakimi, A, Ohashi, M, Akimoto, S, Nishiyama, Y & Asano, Y 2006, 'Latent infection of human herpesvirus 7 in CD4+ T lymphocytes', Journal of Medical Virology, vol. 78, no. 1, pp. 112-116. https://doi.org/10.1002/jmv.20511

Latent infection of human herpesvirus 7 in CD4+ T lymphocytes. / Miyake, Fumi; Yoshikawa, Tetsushi; Sun, He; Kakimi, Akira; Ohashi, Masahiro; Akimoto, Shiho; Nishiyama, Yukihiro; Asano, Yoshizo.

In: Journal of Medical Virology, Vol. 78, No. 1, 01.01.2006, p. 112-116.

Research output: Contribution to journalArticle

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AU - Miyake, Fumi

AU - Yoshikawa, Tetsushi

AU - Sun, He

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AU - Nishiyama, Yukihiro

AU - Asano, Yoshizo

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