Layer-specific sulfatide localization in rat hippocampus middle molecular layer is revealed by nanoparticle-assisted laser desorption/ionization imaging mass spectrometry

Hiroshi Ageta, Sayaka Asai, Yuki Sugiura, Naoko Goto-Inoue, Nobuhiro Zaima, Mitsutoshi Setou

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Lipids are major structural component of the brain and play key roles in signaling functions in the central nervous system (CNS), such as the hippocampus. In particular, sulfatide is an abundant glycosphingolipid component of both the central and the peripheral nervous system and is an essential lipid component of myelin membranes. Lack of sulfatide is observed in myelin deformation and neurological deficits. Previous studies with antisulfatide antibody have investigated distribution of sulfatide expression in neurons; however, this method cannot distinguish the differences of sulfatide lipid species raised by difference of carbon-chain length in the ceramide portion in addition to the differences of sulfatide and seminolipid. In this study, we solved the problem by our recently developed nanoparticle-assisted laser desorption/ionization (nano-PALDI)-based imaging mass spectrometry (IMS). We revealed that the level of sulfatide in the middle molecular layer was significantly higher than that in granule cell layers and the inner molecular layer in the dentate gyrus of rat hippocampus.

Original languageEnglish
Pages (from-to)16-23
Number of pages8
JournalMedical Molecular Morphology
Volume42
Issue number1
DOIs
Publication statusPublished - 01-03-2009

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Sulfoglycosphingolipids
Nanoparticles
Hippocampus
Mass Spectrometry
Lasers
Myelin Sheath
Lipids
Central Nervous System
Glycosphingolipids
Parahippocampal Gyrus
Ceramides
Dentate Gyrus
Peripheral Nervous System
Carbon
Neurons
Membranes
Antibodies
Brain

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology

Cite this

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abstract = "Lipids are major structural component of the brain and play key roles in signaling functions in the central nervous system (CNS), such as the hippocampus. In particular, sulfatide is an abundant glycosphingolipid component of both the central and the peripheral nervous system and is an essential lipid component of myelin membranes. Lack of sulfatide is observed in myelin deformation and neurological deficits. Previous studies with antisulfatide antibody have investigated distribution of sulfatide expression in neurons; however, this method cannot distinguish the differences of sulfatide lipid species raised by difference of carbon-chain length in the ceramide portion in addition to the differences of sulfatide and seminolipid. In this study, we solved the problem by our recently developed nanoparticle-assisted laser desorption/ionization (nano-PALDI)-based imaging mass spectrometry (IMS). We revealed that the level of sulfatide in the middle molecular layer was significantly higher than that in granule cell layers and the inner molecular layer in the dentate gyrus of rat hippocampus.",
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Layer-specific sulfatide localization in rat hippocampus middle molecular layer is revealed by nanoparticle-assisted laser desorption/ionization imaging mass spectrometry. / Ageta, Hiroshi; Asai, Sayaka; Sugiura, Yuki; Goto-Inoue, Naoko; Zaima, Nobuhiro; Setou, Mitsutoshi.

In: Medical Molecular Morphology, Vol. 42, No. 1, 01.03.2009, p. 16-23.

Research output: Contribution to journalArticle

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AU - Ageta, Hiroshi

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AU - Zaima, Nobuhiro

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