Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system

D. Kuboyama; D. Onoshima; N. Kihara; H. Tanaka; T. Hase; H. Yukawa; K. Ishikawa; H. Odaka; Y. Hasegawa; M. Hori; Y. Baba

Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system

D. Kuboyama
, D. Onoshima
, N. Kihara
, H. Tanaka
, T. Hase
, H. Yukawa
, K. Ishikawa
, H. Odaka
, Y. Hasegawa
, M. Hori
, Y. Baba

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present a study of blood microfiltration system towards optimal approach of non-invasive liquid biopsy for cancer detection by circulating tumor cells (CTCs). Filtration behavior of a microfilter was experimentally analyzed for tumor cell isolation from leukocytes in whole blood. This approach achieved an average of >96% recovery of spiked tumor cells and >99% total leukocytes depletion.

Original language	English
Title of host publication	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Publisher	Chemical and Biological Microsystems Society
Pages	882-883
Number of pages	2
ISBN (Electronic)	9780692941836
Publication status	Published - 2020
Externally published	Yes
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States Duration: 22-10-2017 → 26-10-2017

Publication series

Name	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/Territory	United States
City	Savannah
Period	22-10-17 → 26-10-17

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Kuboyama, D., Onoshima, D., Kihara, N., Tanaka, H., Hase, T., Yukawa, H., Ishikawa, K., Odaka, H., Hasegawa, Y., Hori, M., & Baba, Y. (2020). Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 882-883). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.

Kuboyama, D. ; Onoshima, D. ; Kihara, N. et al. / Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2020. pp. 882-883 (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

@inproceedings{894901f7ae4b43999807b9d62bccf3cd,

title = "Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system",

abstract = "We present a study of blood microfiltration system towards optimal approach of non-invasive liquid biopsy for cancer detection by circulating tumor cells (CTCs). Filtration behavior of a microfilter was experimentally analyzed for tumor cell isolation from leukocytes in whole blood. This approach achieved an average of >96\% recovery of spiked tumor cells and >99\% total leukocytes depletion.",

keywords = "Circulating Tumor Cells, Liquid Biopsy, Microfiltration, Open Hole, Single Cell",

author = "D. Kuboyama and D. Onoshima and N. Kihara and H. Tanaka and T. Hase and H. Yukawa and K. Ishikawa and H. Odaka and Y. Hasegawa and M. Hori and Y. Baba",

note = "Publisher Copyright: {\textcopyright} 17CBMS-0001.; 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 ; Conference date: 22-10-2017 Through 26-10-2017",

year = "2020",

language = "English",

series = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",

publisher = "Chemical and Biological Microsystems Society",

pages = "882--883",

booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",

}

Kuboyama, D, Onoshima, D, Kihara, N, Tanaka, H, Hase, T, Yukawa, H, Ishikawa, K, Odaka, H, Hasegawa, Y, Hori, M & Baba, Y 2020, Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system. in 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Chemical and Biological Microsystems Society, pp. 882-883, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Savannah, United States, 22-10-17.

Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system. / Kuboyama, D.; Onoshima, D.; Kihara, N. et al.
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2020. p. 882-883 (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system

AU - Kuboyama, D.

AU - Onoshima, D.

AU - Kihara, N.

AU - Tanaka, H.

AU - Hase, T.

AU - Yukawa, H.

AU - Ishikawa, K.

AU - Odaka, H.

AU - Hasegawa, Y.

AU - Hori, M.

AU - Baba, Y.

PY - 2020

Y1 - 2020

N2 - We present a study of blood microfiltration system towards optimal approach of non-invasive liquid biopsy for cancer detection by circulating tumor cells (CTCs). Filtration behavior of a microfilter was experimentally analyzed for tumor cell isolation from leukocytes in whole blood. This approach achieved an average of >96% recovery of spiked tumor cells and >99% total leukocytes depletion.

AB - We present a study of blood microfiltration system towards optimal approach of non-invasive liquid biopsy for cancer detection by circulating tumor cells (CTCs). Filtration behavior of a microfilter was experimentally analyzed for tumor cell isolation from leukocytes in whole blood. This approach achieved an average of >96% recovery of spiked tumor cells and >99% total leukocytes depletion.

KW - Circulating Tumor Cells

KW - Liquid Biopsy

KW - Microfiltration

KW - Open Hole

KW - Single Cell

UR - https://www.scopus.com/pages/publications/85079613492

UR - https://www.scopus.com/pages/publications/85079613492#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85079613492

T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

SP - 882

EP - 883

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

PB - Chemical and Biological Microsystems Society

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Y2 - 22 October 2017 through 26 October 2017

ER -

Kuboyama D, Onoshima D, Kihara N, Tanaka H, Hase T, Yukawa H et al. Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society. 2020. p. 882-883. (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system

Abstract

Publication series

Conference

UN SDGs

All Science Journal Classification (ASJC) codes

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Cite this