Levels of chondroitin sulfate isomers in synovial fluid of patients with hip osteoarthritis

Harumoto Yamada, Satoshi Miyauchi, Hiraku Hotta, Mitsuhiro Morita, Yasuo Yoshihara, Toshiyuki Kikuchi, Kyosuke Fujikawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We determined the levels of chondroitin sulfate (CS) isomers in the synovial fluid of patients with hip osteoarthritis (OA) to investigate their significance as markers reflecting extracellular matrix metabolism in joint tissues. A cross-sectional study of synovial fluid aspirated from 50 hip joints of 50 patients with OA was performed. Concentrations of chondroitin-4- sulfate (C4S) and chondroitin-6-sulfate (C6S) were determined by high- performance liquid chromatography. Levels of each marker molecule were investigated in relation to age and radiological stage of the disease. In all disease stages, the dominant CS isomer in synovial fluid was C6S. There was a significant negative correlation between levels of C6S and age. A significant inverse correlation was also observed between the ratio of C6S to C4S and age. Results of analysis of covariance in which age was covariate showed that the ratio of C6S to C4S in advanced and terminal stage OA was lower than that in early stage OA. The present results indicate that the ratio of CS isomers in synovial fluid in hip OA varies with the severity of disease. These molecules in synovial fluid may serve as a useful marker reflecting extracellular matrix metabolism in OA.

Original languageEnglish
Pages (from-to)250-254
Number of pages5
JournalJournal of Orthopaedic Science
Volume4
Issue number4
DOIs
Publication statusPublished - 01-01-1999

Fingerprint

Hip Osteoarthritis
Chondroitin Sulfates
Synovial Fluid
Osteoarthritis
Extracellular Matrix
Hip Joint
Cross-Sectional Studies
Joints
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

Yamada, Harumoto ; Miyauchi, Satoshi ; Hotta, Hiraku ; Morita, Mitsuhiro ; Yoshihara, Yasuo ; Kikuchi, Toshiyuki ; Fujikawa, Kyosuke. / Levels of chondroitin sulfate isomers in synovial fluid of patients with hip osteoarthritis. In: Journal of Orthopaedic Science. 1999 ; Vol. 4, No. 4. pp. 250-254.
@article{4f87002aefd9449da5476a2cdf3cb3b2,
title = "Levels of chondroitin sulfate isomers in synovial fluid of patients with hip osteoarthritis",
abstract = "We determined the levels of chondroitin sulfate (CS) isomers in the synovial fluid of patients with hip osteoarthritis (OA) to investigate their significance as markers reflecting extracellular matrix metabolism in joint tissues. A cross-sectional study of synovial fluid aspirated from 50 hip joints of 50 patients with OA was performed. Concentrations of chondroitin-4- sulfate (C4S) and chondroitin-6-sulfate (C6S) were determined by high- performance liquid chromatography. Levels of each marker molecule were investigated in relation to age and radiological stage of the disease. In all disease stages, the dominant CS isomer in synovial fluid was C6S. There was a significant negative correlation between levels of C6S and age. A significant inverse correlation was also observed between the ratio of C6S to C4S and age. Results of analysis of covariance in which age was covariate showed that the ratio of C6S to C4S in advanced and terminal stage OA was lower than that in early stage OA. The present results indicate that the ratio of CS isomers in synovial fluid in hip OA varies with the severity of disease. These molecules in synovial fluid may serve as a useful marker reflecting extracellular matrix metabolism in OA.",
author = "Harumoto Yamada and Satoshi Miyauchi and Hiraku Hotta and Mitsuhiro Morita and Yasuo Yoshihara and Toshiyuki Kikuchi and Kyosuke Fujikawa",
year = "1999",
month = "1",
day = "1",
doi = "10.1007/s007760050100",
language = "English",
volume = "4",
pages = "250--254",
journal = "Journal of Orthopaedic Science",
issn = "0949-2658",
publisher = "Springer Japan",
number = "4",

}

Yamada, H, Miyauchi, S, Hotta, H, Morita, M, Yoshihara, Y, Kikuchi, T & Fujikawa, K 1999, 'Levels of chondroitin sulfate isomers in synovial fluid of patients with hip osteoarthritis', Journal of Orthopaedic Science, vol. 4, no. 4, pp. 250-254. https://doi.org/10.1007/s007760050100

Levels of chondroitin sulfate isomers in synovial fluid of patients with hip osteoarthritis. / Yamada, Harumoto; Miyauchi, Satoshi; Hotta, Hiraku; Morita, Mitsuhiro; Yoshihara, Yasuo; Kikuchi, Toshiyuki; Fujikawa, Kyosuke.

In: Journal of Orthopaedic Science, Vol. 4, No. 4, 01.01.1999, p. 250-254.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Levels of chondroitin sulfate isomers in synovial fluid of patients with hip osteoarthritis

AU - Yamada, Harumoto

AU - Miyauchi, Satoshi

AU - Hotta, Hiraku

AU - Morita, Mitsuhiro

AU - Yoshihara, Yasuo

AU - Kikuchi, Toshiyuki

AU - Fujikawa, Kyosuke

PY - 1999/1/1

Y1 - 1999/1/1

N2 - We determined the levels of chondroitin sulfate (CS) isomers in the synovial fluid of patients with hip osteoarthritis (OA) to investigate their significance as markers reflecting extracellular matrix metabolism in joint tissues. A cross-sectional study of synovial fluid aspirated from 50 hip joints of 50 patients with OA was performed. Concentrations of chondroitin-4- sulfate (C4S) and chondroitin-6-sulfate (C6S) were determined by high- performance liquid chromatography. Levels of each marker molecule were investigated in relation to age and radiological stage of the disease. In all disease stages, the dominant CS isomer in synovial fluid was C6S. There was a significant negative correlation between levels of C6S and age. A significant inverse correlation was also observed between the ratio of C6S to C4S and age. Results of analysis of covariance in which age was covariate showed that the ratio of C6S to C4S in advanced and terminal stage OA was lower than that in early stage OA. The present results indicate that the ratio of CS isomers in synovial fluid in hip OA varies with the severity of disease. These molecules in synovial fluid may serve as a useful marker reflecting extracellular matrix metabolism in OA.

AB - We determined the levels of chondroitin sulfate (CS) isomers in the synovial fluid of patients with hip osteoarthritis (OA) to investigate their significance as markers reflecting extracellular matrix metabolism in joint tissues. A cross-sectional study of synovial fluid aspirated from 50 hip joints of 50 patients with OA was performed. Concentrations of chondroitin-4- sulfate (C4S) and chondroitin-6-sulfate (C6S) were determined by high- performance liquid chromatography. Levels of each marker molecule were investigated in relation to age and radiological stage of the disease. In all disease stages, the dominant CS isomer in synovial fluid was C6S. There was a significant negative correlation between levels of C6S and age. A significant inverse correlation was also observed between the ratio of C6S to C4S and age. Results of analysis of covariance in which age was covariate showed that the ratio of C6S to C4S in advanced and terminal stage OA was lower than that in early stage OA. The present results indicate that the ratio of CS isomers in synovial fluid in hip OA varies with the severity of disease. These molecules in synovial fluid may serve as a useful marker reflecting extracellular matrix metabolism in OA.

UR - http://www.scopus.com/inward/record.url?scp=0032806677&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032806677&partnerID=8YFLogxK

U2 - 10.1007/s007760050100

DO - 10.1007/s007760050100

M3 - Article

C2 - 10436271

AN - SCOPUS:0032806677

VL - 4

SP - 250

EP - 254

JO - Journal of Orthopaedic Science

JF - Journal of Orthopaedic Science

SN - 0949-2658

IS - 4

ER -