Lipid-Mediated Transbronchial Human Interleukin-10 Gene Transfer Decreases Acute Inflammation Associated With Allograft Rejection in a Rat Model of Lung Transplantation

H. Oishi, Y. Okada, T. Kikuchi, T. Sado, T. Oyaizu, Yasushi Hoshikawa, S. Suzuki, Y. Matsumura, T. Kondo

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Abstract

Background: Transferring genes with immunoregulatory capacity to transplanted organs has the potential to modify allograft rejection (AR). We examined the effect of ex vivo lipid-mediated transbronchial human interleukin-10 (hIL-10) gene transfer on acute AR in a rat model of lung transplantation. Methods: Left single lung transplantations were performed between a highly histoincompatible rat combination: Brown Norway to Lewis. The extracted donor left lung was intrabronchially instilled with a plasmid encoding hIL-10 or Escherichia coli β-galactosidase (control), mixed with a cationic lipid. On day 6 posttransplantation, the degree of AR was graded histologically (stages 1-4) based upon pathological categories of inflammation: perivascular, peribronchial, and peribronchiolar lymphocytic infiltrates, edema, intraalveolar hemorrhage, and necrosis. Results: The stage of AR in the IL-10 group (3.1 ± 0.4) was significantly lower than the control group (3.8 ± 0.4). Pathological scores for edema, intraalveolar hemorrhage, and necrosis in the IL-10 group (2.3 ± 0.8, 0.3 ± 0.5, and 0.3 ± 0.5, respectively) were also significantly decreased compared with those in the control group (3.2 ± 0.4, 2.2 ± 0.8, and 1.2 ± 0.4, respectively). Conclusion: Ex vivo lipid-mediated transbronchial hIL-10 gene transfer attenuated acute inflammation associated with AR in a rat model of lung transplantation.

Original languageEnglish
Pages (from-to)283-285
Number of pages3
JournalTransplantation Proceedings
Volume39
Issue number1
DOIs
Publication statusPublished - 01-01-2007

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Lung Transplantation
Interleukin-10
Allografts
Inflammation
Lipids
Genes
Edema
Necrosis
Galactosidases
Hemorrhage
Control Groups
Norway
Plasmids
Escherichia coli
Lung

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Oishi, H. ; Okada, Y. ; Kikuchi, T. ; Sado, T. ; Oyaizu, T. ; Hoshikawa, Yasushi ; Suzuki, S. ; Matsumura, Y. ; Kondo, T. / Lipid-Mediated Transbronchial Human Interleukin-10 Gene Transfer Decreases Acute Inflammation Associated With Allograft Rejection in a Rat Model of Lung Transplantation. In: Transplantation Proceedings. 2007 ; Vol. 39, No. 1. pp. 283-285.
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title = "Lipid-Mediated Transbronchial Human Interleukin-10 Gene Transfer Decreases Acute Inflammation Associated With Allograft Rejection in a Rat Model of Lung Transplantation",
abstract = "Background: Transferring genes with immunoregulatory capacity to transplanted organs has the potential to modify allograft rejection (AR). We examined the effect of ex vivo lipid-mediated transbronchial human interleukin-10 (hIL-10) gene transfer on acute AR in a rat model of lung transplantation. Methods: Left single lung transplantations were performed between a highly histoincompatible rat combination: Brown Norway to Lewis. The extracted donor left lung was intrabronchially instilled with a plasmid encoding hIL-10 or Escherichia coli β-galactosidase (control), mixed with a cationic lipid. On day 6 posttransplantation, the degree of AR was graded histologically (stages 1-4) based upon pathological categories of inflammation: perivascular, peribronchial, and peribronchiolar lymphocytic infiltrates, edema, intraalveolar hemorrhage, and necrosis. Results: The stage of AR in the IL-10 group (3.1 ± 0.4) was significantly lower than the control group (3.8 ± 0.4). Pathological scores for edema, intraalveolar hemorrhage, and necrosis in the IL-10 group (2.3 ± 0.8, 0.3 ± 0.5, and 0.3 ± 0.5, respectively) were also significantly decreased compared with those in the control group (3.2 ± 0.4, 2.2 ± 0.8, and 1.2 ± 0.4, respectively). Conclusion: Ex vivo lipid-mediated transbronchial hIL-10 gene transfer attenuated acute inflammation associated with AR in a rat model of lung transplantation.",
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Lipid-Mediated Transbronchial Human Interleukin-10 Gene Transfer Decreases Acute Inflammation Associated With Allograft Rejection in a Rat Model of Lung Transplantation. / Oishi, H.; Okada, Y.; Kikuchi, T.; Sado, T.; Oyaizu, T.; Hoshikawa, Yasushi; Suzuki, S.; Matsumura, Y.; Kondo, T.

In: Transplantation Proceedings, Vol. 39, No. 1, 01.01.2007, p. 283-285.

Research output: Contribution to journalArticle

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T1 - Lipid-Mediated Transbronchial Human Interleukin-10 Gene Transfer Decreases Acute Inflammation Associated With Allograft Rejection in a Rat Model of Lung Transplantation

AU - Oishi, H.

AU - Okada, Y.

AU - Kikuchi, T.

AU - Sado, T.

AU - Oyaizu, T.

AU - Hoshikawa, Yasushi

AU - Suzuki, S.

AU - Matsumura, Y.

AU - Kondo, T.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Background: Transferring genes with immunoregulatory capacity to transplanted organs has the potential to modify allograft rejection (AR). We examined the effect of ex vivo lipid-mediated transbronchial human interleukin-10 (hIL-10) gene transfer on acute AR in a rat model of lung transplantation. Methods: Left single lung transplantations were performed between a highly histoincompatible rat combination: Brown Norway to Lewis. The extracted donor left lung was intrabronchially instilled with a plasmid encoding hIL-10 or Escherichia coli β-galactosidase (control), mixed with a cationic lipid. On day 6 posttransplantation, the degree of AR was graded histologically (stages 1-4) based upon pathological categories of inflammation: perivascular, peribronchial, and peribronchiolar lymphocytic infiltrates, edema, intraalveolar hemorrhage, and necrosis. Results: The stage of AR in the IL-10 group (3.1 ± 0.4) was significantly lower than the control group (3.8 ± 0.4). Pathological scores for edema, intraalveolar hemorrhage, and necrosis in the IL-10 group (2.3 ± 0.8, 0.3 ± 0.5, and 0.3 ± 0.5, respectively) were also significantly decreased compared with those in the control group (3.2 ± 0.4, 2.2 ± 0.8, and 1.2 ± 0.4, respectively). Conclusion: Ex vivo lipid-mediated transbronchial hIL-10 gene transfer attenuated acute inflammation associated with AR in a rat model of lung transplantation.

AB - Background: Transferring genes with immunoregulatory capacity to transplanted organs has the potential to modify allograft rejection (AR). We examined the effect of ex vivo lipid-mediated transbronchial human interleukin-10 (hIL-10) gene transfer on acute AR in a rat model of lung transplantation. Methods: Left single lung transplantations were performed between a highly histoincompatible rat combination: Brown Norway to Lewis. The extracted donor left lung was intrabronchially instilled with a plasmid encoding hIL-10 or Escherichia coli β-galactosidase (control), mixed with a cationic lipid. On day 6 posttransplantation, the degree of AR was graded histologically (stages 1-4) based upon pathological categories of inflammation: perivascular, peribronchial, and peribronchiolar lymphocytic infiltrates, edema, intraalveolar hemorrhage, and necrosis. Results: The stage of AR in the IL-10 group (3.1 ± 0.4) was significantly lower than the control group (3.8 ± 0.4). Pathological scores for edema, intraalveolar hemorrhage, and necrosis in the IL-10 group (2.3 ± 0.8, 0.3 ± 0.5, and 0.3 ± 0.5, respectively) were also significantly decreased compared with those in the control group (3.2 ± 0.4, 2.2 ± 0.8, and 1.2 ± 0.4, respectively). Conclusion: Ex vivo lipid-mediated transbronchial hIL-10 gene transfer attenuated acute inflammation associated with AR in a rat model of lung transplantation.

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