Lipopolysaccharide induction of indoleamine 2,3-dioxygenase is mediated dominantly by an IFN-γ-independent mechanism

Suwako Fujigaki, Kuniaki Saito, Kenji Sekikawa, Shigenobu Tone, Osamu Takikawa, Hidehiko Fujii, Hisayasu Wada, Akio Noma, Mitsuru Seishima

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Indoleamine 2,3-dioxygenase (IDO) is a rate-limiting enzyme in the L-tryptophan-kynurenine pathway, which converts an essential amino acid, L-tryptophan, to N-formylkynurenine. It has been speculated that IFN-γ is a dominant IDO inducer in vivo. The present study used IFN-γ or TNF-α gene-disrupted mice and IFN-γ antibody-treated mice to demonstrate that lipopolysaccharide (LPS)-induced systemic IDO is largely dependent on TNF-α rather than IFN-γ. IFN-γ-independent IDO induction was also demonstrated in vitro with LPS-stimulated monocytic THP-1 cells. These findings clearly indicate that there is an IFN-γ-independent mechanism of IDO induction in addition to the IFN-γ-dependent mechanism.

Original languageEnglish
Pages (from-to)2313-2318
Number of pages6
JournalEuropean Journal of Immunology
Volume31
Issue number8
DOIs
Publication statusPublished - 06-09-2001

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Lipopolysaccharides
Tryptophan
Kynurenine
Essential Amino Acids
Antibodies
Enzymes
Genes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Fujigaki, Suwako ; Saito, Kuniaki ; Sekikawa, Kenji ; Tone, Shigenobu ; Takikawa, Osamu ; Fujii, Hidehiko ; Wada, Hisayasu ; Noma, Akio ; Seishima, Mitsuru. / Lipopolysaccharide induction of indoleamine 2,3-dioxygenase is mediated dominantly by an IFN-γ-independent mechanism. In: European Journal of Immunology. 2001 ; Vol. 31, No. 8. pp. 2313-2318.
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Lipopolysaccharide induction of indoleamine 2,3-dioxygenase is mediated dominantly by an IFN-γ-independent mechanism. / Fujigaki, Suwako; Saito, Kuniaki; Sekikawa, Kenji; Tone, Shigenobu; Takikawa, Osamu; Fujii, Hidehiko; Wada, Hisayasu; Noma, Akio; Seishima, Mitsuru.

In: European Journal of Immunology, Vol. 31, No. 8, 06.09.2001, p. 2313-2318.

Research output: Contribution to journalArticle

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AU - Fujigaki, Suwako

AU - Saito, Kuniaki

AU - Sekikawa, Kenji

AU - Tone, Shigenobu

AU - Takikawa, Osamu

AU - Fujii, Hidehiko

AU - Wada, Hisayasu

AU - Noma, Akio

AU - Seishima, Mitsuru

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