Lipopolysaccharide prevents apoptosis induced by brefeldin A, an endoplasmic reticulum stress agent, in RAW 264.7 cells

Shamima Islam, Ferdaus Hassan, Gantsetseg Tumurkhuu, Hiroyasu Ito, Naoki Koide, Isamu Mori, Tomoaki Yoshida, Takashi Yokochi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of lipopolysaccharide (LPS) on the cell death induced by endoplasmic reticulum (ER) stress agents in RAW 264.7 cells was studied. LPS prevented the cell death by brefeldin A, but not thapsigargin and tunicamycin. CpG DNA as well as LPS prevented brefeldin A-induced cell death whereas tumor necrosis factor-α or interferon-γ did not. Brefeldin A-induced cell death was mediated with apoptotic cell death and it was significantly inhibited by LPS. LPS abolished the activation of ER stress-related caspases, such as caspases 1, 3, and 4. LPS prevented brefeldin A-induced morphological changes in RAW 264.7 cells. Further, LPS prevented brefeldin A-induced Golgi dispersion. Therefore, LPS was suggested to diminish the stress of ER/Golgi complexes induced by brefeldin A and inhibit apoptosis. The preventive action of LPS on brefeldin A-induced apoptosis is discussed.

Original languageEnglish
Pages (from-to)589-596
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume340
Issue number2
DOIs
Publication statusPublished - 10-02-2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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