@inbook{f4df3651f91e463aacef0e66f13a20af,
title = "Live imaging of fetal intra-abdominal organs using two-photon laser-scanning microscopy",
abstract = "The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord.",
author = "Yuhki Koike and Bo Li and Yong Chen and Hiromu Miyake and Carol Lee and Lijun Chi and Richard Wu and Mikihiro Inoue and Keiichi Uchida and Masato Kusunoki and Paul Delgado-Olguin and Agostino Pierro",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-7714-7\_6",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "63--69",
booktitle = "Methods in Molecular Biology",
}