Liver transplantation from female donors provokes higher complement component C5a activity

Mohamed Hamed Hussein, Takashi Hashimoto, Tatsuya Suzuki, Ghada Abdel Hamid Daoud, Takazumi Kato, Masahito Hibi, Hirokazu Tomishige, Fujio Hara, Tetsuya Ito, Ineko Kato, Kabe Kazuhiko, Hisanori Sobajima, Masanori Tamura, Hajime Togari

Research output: Contribution to journalArticle

Abstract

Background: Transplanted organs from female donors are associated with less favorable prognoses and outcomes. This study aimed to determine whether donor gender affects levels of serum terminal complement component C5a and oxidative stress in pediatric living related liver transplantation (LRLTx) recipients. Material/Methods: The subjects were 43 patients (20 males and 23 females) who underwent LRLTx during childhood (age range 1.2 years to 14.4 years; mean age 5 years). Serum samples were taken during the patients’ regular outpatient visits after LRLTx. Serum C5a was measured using the specific human C5a ELISA kit. Serum total hydroperoxide (TH) and biological antioxidative potential (BAP) were measured using the free radical analytic system, and the oxidative stress index (OSI) was calculated as the ratio of TH to BAP. Serum glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), gamma-glutamyl transpeptidase (γGTP), and lactate dehydrogenase (LDH) were also measured as part of a typical outpatient examination for such patients. Results: C5a serum levels were higher in the 29 recipients who received their grafted livers from female donors than in the 14 recipients who received their grafted livers from male donors. Recipients who received their grafted livers from female donors had higher incidence of post-LTx (liver transplantation) complications. Female recipients from female donors showed the highest serum GPT and GOT levels, but this difference was only significant when compared to the female recipients from male donors (41.4±9.8 IU/L vs. 17.3±1.8 IU/L for GPT and 42.2±7.5 IU/L vs. 23.4±2.2 IU/L for GOT; P<0.05). Conclusions: Pediatric LRLTx patients who receive their grafts from female donors exhibit higher levels of serum C5a that probably plays a role in the immunological response against grafted livers from female donors in LTx.

Original languageEnglish
Pages (from-to)694-700
Number of pages7
JournalAnnals of Transplantation
Volume22
DOIs
Publication statusPublished - 01-01-2017

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Complement C5a
Liver Transplantation
Tissue Donors
Serum
Aspartate Aminotransferases
Alanine Transaminase
Liver
Hydrogen Peroxide
Oxidative Stress
Outpatients
Pediatrics
gamma-Glutamyltransferase
L-Lactate Dehydrogenase
Free Radicals
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Transplantation

Cite this

Hussein, M. H., Hashimoto, T., Suzuki, T., Daoud, G. A. H., Kato, T., Hibi, M., ... Togari, H. (2017). Liver transplantation from female donors provokes higher complement component C5a activity. Annals of Transplantation, 22, 694-700. https://doi.org/10.12659/AOT.905464
Hussein, Mohamed Hamed ; Hashimoto, Takashi ; Suzuki, Tatsuya ; Daoud, Ghada Abdel Hamid ; Kato, Takazumi ; Hibi, Masahito ; Tomishige, Hirokazu ; Hara, Fujio ; Ito, Tetsuya ; Kato, Ineko ; Kazuhiko, Kabe ; Sobajima, Hisanori ; Tamura, Masanori ; Togari, Hajime. / Liver transplantation from female donors provokes higher complement component C5a activity. In: Annals of Transplantation. 2017 ; Vol. 22. pp. 694-700.
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abstract = "Background: Transplanted organs from female donors are associated with less favorable prognoses and outcomes. This study aimed to determine whether donor gender affects levels of serum terminal complement component C5a and oxidative stress in pediatric living related liver transplantation (LRLTx) recipients. Material/Methods: The subjects were 43 patients (20 males and 23 females) who underwent LRLTx during childhood (age range 1.2 years to 14.4 years; mean age 5 years). Serum samples were taken during the patients’ regular outpatient visits after LRLTx. Serum C5a was measured using the specific human C5a ELISA kit. Serum total hydroperoxide (TH) and biological antioxidative potential (BAP) were measured using the free radical analytic system, and the oxidative stress index (OSI) was calculated as the ratio of TH to BAP. Serum glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), gamma-glutamyl transpeptidase (γGTP), and lactate dehydrogenase (LDH) were also measured as part of a typical outpatient examination for such patients. Results: C5a serum levels were higher in the 29 recipients who received their grafted livers from female donors than in the 14 recipients who received their grafted livers from male donors. Recipients who received their grafted livers from female donors had higher incidence of post-LTx (liver transplantation) complications. Female recipients from female donors showed the highest serum GPT and GOT levels, but this difference was only significant when compared to the female recipients from male donors (41.4±9.8 IU/L vs. 17.3±1.8 IU/L for GPT and 42.2±7.5 IU/L vs. 23.4±2.2 IU/L for GOT; P<0.05). Conclusions: Pediatric LRLTx patients who receive their grafts from female donors exhibit higher levels of serum C5a that probably plays a role in the immunological response against grafted livers from female donors in LTx.",
author = "Hussein, {Mohamed Hamed} and Takashi Hashimoto and Tatsuya Suzuki and Daoud, {Ghada Abdel Hamid} and Takazumi Kato and Masahito Hibi and Hirokazu Tomishige and Fujio Hara and Tetsuya Ito and Ineko Kato and Kabe Kazuhiko and Hisanori Sobajima and Masanori Tamura and Hajime Togari",
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Hussein, MH, Hashimoto, T, Suzuki, T, Daoud, GAH, Kato, T, Hibi, M, Tomishige, H, Hara, F, Ito, T, Kato, I, Kazuhiko, K, Sobajima, H, Tamura, M & Togari, H 2017, 'Liver transplantation from female donors provokes higher complement component C5a activity', Annals of Transplantation, vol. 22, pp. 694-700. https://doi.org/10.12659/AOT.905464

Liver transplantation from female donors provokes higher complement component C5a activity. / Hussein, Mohamed Hamed; Hashimoto, Takashi; Suzuki, Tatsuya; Daoud, Ghada Abdel Hamid; Kato, Takazumi; Hibi, Masahito; Tomishige, Hirokazu; Hara, Fujio; Ito, Tetsuya; Kato, Ineko; Kazuhiko, Kabe; Sobajima, Hisanori; Tamura, Masanori; Togari, Hajime.

In: Annals of Transplantation, Vol. 22, 01.01.2017, p. 694-700.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Liver transplantation from female donors provokes higher complement component C5a activity

AU - Hussein, Mohamed Hamed

AU - Hashimoto, Takashi

AU - Suzuki, Tatsuya

AU - Daoud, Ghada Abdel Hamid

AU - Kato, Takazumi

AU - Hibi, Masahito

AU - Tomishige, Hirokazu

AU - Hara, Fujio

AU - Ito, Tetsuya

AU - Kato, Ineko

AU - Kazuhiko, Kabe

AU - Sobajima, Hisanori

AU - Tamura, Masanori

AU - Togari, Hajime

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: Transplanted organs from female donors are associated with less favorable prognoses and outcomes. This study aimed to determine whether donor gender affects levels of serum terminal complement component C5a and oxidative stress in pediatric living related liver transplantation (LRLTx) recipients. Material/Methods: The subjects were 43 patients (20 males and 23 females) who underwent LRLTx during childhood (age range 1.2 years to 14.4 years; mean age 5 years). Serum samples were taken during the patients’ regular outpatient visits after LRLTx. Serum C5a was measured using the specific human C5a ELISA kit. Serum total hydroperoxide (TH) and biological antioxidative potential (BAP) were measured using the free radical analytic system, and the oxidative stress index (OSI) was calculated as the ratio of TH to BAP. Serum glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), gamma-glutamyl transpeptidase (γGTP), and lactate dehydrogenase (LDH) were also measured as part of a typical outpatient examination for such patients. Results: C5a serum levels were higher in the 29 recipients who received their grafted livers from female donors than in the 14 recipients who received their grafted livers from male donors. Recipients who received their grafted livers from female donors had higher incidence of post-LTx (liver transplantation) complications. Female recipients from female donors showed the highest serum GPT and GOT levels, but this difference was only significant when compared to the female recipients from male donors (41.4±9.8 IU/L vs. 17.3±1.8 IU/L for GPT and 42.2±7.5 IU/L vs. 23.4±2.2 IU/L for GOT; P<0.05). Conclusions: Pediatric LRLTx patients who receive their grafts from female donors exhibit higher levels of serum C5a that probably plays a role in the immunological response against grafted livers from female donors in LTx.

AB - Background: Transplanted organs from female donors are associated with less favorable prognoses and outcomes. This study aimed to determine whether donor gender affects levels of serum terminal complement component C5a and oxidative stress in pediatric living related liver transplantation (LRLTx) recipients. Material/Methods: The subjects were 43 patients (20 males and 23 females) who underwent LRLTx during childhood (age range 1.2 years to 14.4 years; mean age 5 years). Serum samples were taken during the patients’ regular outpatient visits after LRLTx. Serum C5a was measured using the specific human C5a ELISA kit. Serum total hydroperoxide (TH) and biological antioxidative potential (BAP) were measured using the free radical analytic system, and the oxidative stress index (OSI) was calculated as the ratio of TH to BAP. Serum glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), gamma-glutamyl transpeptidase (γGTP), and lactate dehydrogenase (LDH) were also measured as part of a typical outpatient examination for such patients. Results: C5a serum levels were higher in the 29 recipients who received their grafted livers from female donors than in the 14 recipients who received their grafted livers from male donors. Recipients who received their grafted livers from female donors had higher incidence of post-LTx (liver transplantation) complications. Female recipients from female donors showed the highest serum GPT and GOT levels, but this difference was only significant when compared to the female recipients from male donors (41.4±9.8 IU/L vs. 17.3±1.8 IU/L for GPT and 42.2±7.5 IU/L vs. 23.4±2.2 IU/L for GOT; P<0.05). Conclusions: Pediatric LRLTx patients who receive their grafts from female donors exhibit higher levels of serum C5a that probably plays a role in the immunological response against grafted livers from female donors in LTx.

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