Localization of epitopes recognized by monoclonal antibodies that neutralized the H3N2 influenza viruses in man

Jun Okada, Nobuko Oshima, Ritsuko Kubota-Koketsu, Yoshitaka Iba, Sayuri Ota, Wakana Takase, Tetsushi Yoshikawa, Toyokazu Ishikawa, Yoshizo Asano, Yoshinobu Okuno, Yoshikazu Kurosawa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Through extensive isolation of neutralizing mAbs against H3N2 influenza viruses representing the in vivo repertoire in a human donor, we examined the relationships between antigenic drift of influenza virus and protective antibodies generated in an infected individual. The majority of mAbs isolated from a donor born in 1960 were divided into three major groups with distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003. In the present study, we developed a new method that allowed us to comprehensively determine the location of epitopes recognized by many mAbs. Original haemagglutinins (HAs) of several strains and chimaeric variants, in which one of the seven sites (A, B1, B2, C1, C2, D or E) was replaced by some other strain-derived sequence, were artificially expressed on the cell surface. The binding activity of mAbs to the HAs was examined by flow cytometry. By using this method, we determined the location of epitopes recognized by 98 different mAbs. Clones that neutralize the 1968-1973 strains bind to site B2/D, A or A/B1. While sites C, E and B were recognized by clones that neutralized the 1977-1993 strains, the majority of these clones bind to site C. Clones that neutralize the 1997-2003 strains bind to site B, A/B1, A/B2 or E/C2.

Original languageEnglish
Pages (from-to)326-335
Number of pages10
JournalJournal of General Virology
Volume92
Issue number2
DOIs
Publication statusPublished - 01-02-2011

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H3N2 Subtype Influenza A Virus
Orthomyxoviridae
Epitopes
Clone Cells
Monoclonal Antibodies
Tissue Donors
Hemagglutinins
Flow Cytometry
Antibodies

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Okada, Jun ; Oshima, Nobuko ; Kubota-Koketsu, Ritsuko ; Iba, Yoshitaka ; Ota, Sayuri ; Takase, Wakana ; Yoshikawa, Tetsushi ; Ishikawa, Toyokazu ; Asano, Yoshizo ; Okuno, Yoshinobu ; Kurosawa, Yoshikazu. / Localization of epitopes recognized by monoclonal antibodies that neutralized the H3N2 influenza viruses in man. In: Journal of General Virology. 2011 ; Vol. 92, No. 2. pp. 326-335.
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Okada, J, Oshima, N, Kubota-Koketsu, R, Iba, Y, Ota, S, Takase, W, Yoshikawa, T, Ishikawa, T, Asano, Y, Okuno, Y & Kurosawa, Y 2011, 'Localization of epitopes recognized by monoclonal antibodies that neutralized the H3N2 influenza viruses in man', Journal of General Virology, vol. 92, no. 2, pp. 326-335. https://doi.org/10.1099/vir.0.026419-0

Localization of epitopes recognized by monoclonal antibodies that neutralized the H3N2 influenza viruses in man. / Okada, Jun; Oshima, Nobuko; Kubota-Koketsu, Ritsuko; Iba, Yoshitaka; Ota, Sayuri; Takase, Wakana; Yoshikawa, Tetsushi; Ishikawa, Toyokazu; Asano, Yoshizo; Okuno, Yoshinobu; Kurosawa, Yoshikazu.

In: Journal of General Virology, Vol. 92, No. 2, 01.02.2011, p. 326-335.

Research output: Contribution to journalArticle

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