Localization of Rabphilin-3A, a Putative Target Protein for Rab3A, at the Sites of Ca2+-Dependent Exocytosis in PC12 Cells

K. Wada, A. Mizoguchi, K. Kaibuchi, H. Shirataki, C. Ide, Y. Takai

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Rab3A/Smg 25A, a small GTP-binding protein, is highly concentrated in presynapse of neurons and implicated in neurotransmitter release. We have recently identified a putative target protein for Rab3A, isolated its cDNA, and designated it as Rabphilin-3A. To examine whether Rabphilin-3A as well as Rab3A is localized at the sites of Ca2+-dependent exocytosis, we investigated here localization of Rabphilin-3A and Rab3A in comparison with the sites of exocytosis ira the differentiated PC12 cells. Rabphilin-3A as well as Rab3A was highly concentrated at the tips of the neurites where Ca2+-dependent exocytosis took place. Inversely, neither Rabphilin-3A nor Rab3A was concentrated at the tips of the neurites where Ca2+-dependent exocytosis did not take place. These results suggest that Rabphilin-3A as well as Rab3A constitutes a part of the machinery necessary for neurotransmitter release.

Original languageEnglish
Pages (from-to)158-165
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume198
Issue number1
DOIs
Publication statusPublished - 15-01-1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Localization of Rabphilin-3A, a Putative Target Protein for Rab3A, at the Sites of Ca<sup>2+</sup>-Dependent Exocytosis in PC12 Cells'. Together they form a unique fingerprint.

Cite this