TY - JOUR
T1 - Localization of the gene coding for myosin phosphatase, target subunit 1 (MYPT1) to human chromosome 12q15-q21
AU - Takahashi, N.
AU - Ito, M.
AU - Tanaka, J.
AU - Nakano, T.
AU - Kaibuchi, K.
AU - Odai, H.
AU - Takemura, K.
N1 - Funding Information:
We thank Dr. H. Saya and Dr. H. Tsuiki for their technical advice on radiation hybrid mapping, Dr. T. Ohtani for advice and support, and Y. Yosioka for technical assistance. This work was supported in part by grants-in-aid for Scienti®c Research, for Cancer Research, and for International Scienti®c Research from the Ministry of Education, Science and Culture, Japan.
PY - 1997/8/15
Y1 - 1997/8/15
N2 - Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the inter-action of actin and myosin downstream of the guanosine triphosphatase Rho, as previously shown (K. Kimura, et aL, 1996, Science 273: 245-248). To understand the role of MYPT1 in the regulation of the cytoskeleton in human diseases, we have cloned a 4855-bp cDNA for human MYPT1 using the rat MYPT1 cDNA as probe. Sequencing analysis has revealed that human MYPT1 contains 1030 amino acid residues with a calculated molecular mass of approximately 115 kDa. Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybrid mapping has shown that the human MYPT1 gene is located very near the highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106.
AB - Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the inter-action of actin and myosin downstream of the guanosine triphosphatase Rho, as previously shown (K. Kimura, et aL, 1996, Science 273: 245-248). To understand the role of MYPT1 in the regulation of the cytoskeleton in human diseases, we have cloned a 4855-bp cDNA for human MYPT1 using the rat MYPT1 cDNA as probe. Sequencing analysis has revealed that human MYPT1 contains 1030 amino acid residues with a calculated molecular mass of approximately 115 kDa. Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybrid mapping has shown that the human MYPT1 gene is located very near the highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106.
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U2 - 10.1006/geno.1997.4859
DO - 10.1006/geno.1997.4859
M3 - Article
C2 - 9286714
AN - SCOPUS:0031571683
SN - 0888-7543
VL - 44
SP - 150
EP - 152
JO - Genomics
JF - Genomics
IS - 1
ER -