Long-spacing collagen in the human corneal stroma

K. Hirano, M. Nakamura, M. Kobayashi, K. Kobayashi, T. Hoshino, S. Awaya

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


An electron microscopic observation of a corneal lenticule removed 14 months after epikeratoplasty because of refractive error and an unused lenticule was carried out to determine the presence of long-spacing collagen. In the removed lenticule, long-spacing collagen, which is often described as the product of the keratocvtes migrating from the host corneal stroma, was observed near a keratocyte. However, long-spacing collagen was also observed in the unused lenticule. In this specimen, keratocytes had been destroyed by freezing so that the secretion of long-spacing collagen by keratocytes should have been negligible. Thus, it seemed that long-spacing collagen could exist naturally in the corneal lenticule as well as be newly formed by migrating keratocytes. In addition, we examined four corneas from patients ranging from 2 months to 91 years of age with no past history of corneal diseases or disorders. Long-spacing collagen was seen in the corneal stromas of the aged persons, but not in those of the younger. In the corneas of the 78-year-and 91-year-old, a different appearance from the usual long-spacing collagen was also seen in the collagen fibrils which showed a slightly shorter periodicity resembling experimentally formed 100 nm periodic fibrils in mice. Long spacing collagen is a common component of normal human corneal stroma and its occurrence seems to correlate with the age-related changes of the tissue.

Original languageEnglish
Pages (from-to)148-155
Number of pages8
JournalJapanese Journal of Ophthalmology
Issue number2
Publication statusPublished - 01-01-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Ophthalmology


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