Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B

Masaru Ihira, Akane Ohta, Ken Sugata, Sadao Suga, Yoshizo Asano, Tetsushi Yoshikawa

Research output: Contribution to journalArticle

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Abstract

Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.

Original languageEnglish
Pages (from-to)223-225
Number of pages3
JournalJournal of Virological Methods
Volume154
Issue number1-2
DOIs
Publication statusPublished - 01-12-2008

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Human Herpesvirus 6
Cercopithecine Herpesvirus 1
Herpesviridae Infections
Agar Gel Electrophoresis
Serum

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Ihira, Masaru ; Ohta, Akane ; Sugata, Ken ; Suga, Sadao ; Asano, Yoshizo ; Yoshikawa, Tetsushi. / Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B. In: Journal of Virological Methods. 2008 ; Vol. 154, No. 1-2. pp. 223-225.
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Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B. / Ihira, Masaru; Ohta, Akane; Sugata, Ken; Suga, Sadao; Asano, Yoshizo; Yoshikawa, Tetsushi.

In: Journal of Virological Methods, Vol. 154, No. 1-2, 01.12.2008, p. 223-225.

Research output: Contribution to journalArticle

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AU - Yoshikawa, Tetsushi

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AB - Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.

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