TY - JOUR
T1 - Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B
AU - Ihira, Masaru
AU - Ohta, Akane
AU - Sugata, Ken
AU - Suga, Sadao
AU - Asano, Yoshizo
AU - Yoshikawa, Tetsushi
N1 - Funding Information:
Grant support: This work was supported in part by a grant-in-aid for the Open Research Center, and the grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan. This study was also conducted with the support of a grant for Research Promotion of Emerging and Re-emerging Infectious Diseases (H18-Shinko-004) from the Ministry of Health, Labour and Welfare of Japan.
PY - 2008/12
Y1 - 2008/12
N2 - Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.
AB - Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.
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U2 - 10.1016/j.jviromet.2008.07.004
DO - 10.1016/j.jviromet.2008.07.004
M3 - Article
C2 - 18706448
AN - SCOPUS:56249121842
SN - 0166-0934
VL - 154
SP - 223
EP - 225
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1-2
ER -
