Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B

Masaru Ihira; Akane Ohta; Ken Sugata; Sadao Suga; Yoshizo Asano; Tetsushi Yoshikawa

doi:10.1016/j.jviromet.2008.07.004

Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B

Masaru Ihira
, Akane Ohta
, Ken Sugata
, Sadao Suga
, Yoshizo Asano
, Tetsushi Yoshikawa

Clinical Science for biological monitoring

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.

Original language	English
Pages (from-to)	223-225
Number of pages	3
Journal	Journal of Virological Methods
Volume	154
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2008.07.004
Publication status	Published - 12-2008

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Virology

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10.1016/j.jviromet.2008.07.004

Cite this

@article{43fc502a1c1e4dfaa0b05d7ab066c1f8,

title = "Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B",

abstract = "Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.",

keywords = "Genotyping, HHV-6, LAMP, Variant",

author = "Masaru Ihira and Akane Ohta and Ken Sugata and Sadao Suga and Yoshizo Asano and Tetsushi Yoshikawa",

note = "Funding Information: Grant support: This work was supported in part by a grant-in-aid for the Open Research Center, and the grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan. This study was also conducted with the support of a grant for Research Promotion of Emerging and Re-emerging Infectious Diseases (H18-Shinko-004) from the Ministry of Health, Labour and Welfare of Japan.",

year = "2008",

month = dec,

doi = "10.1016/j.jviromet.2008.07.004",

language = "English",

volume = "154",

pages = "223--225",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B

AU - Ihira, Masaru

AU - Ohta, Akane

AU - Sugata, Ken

AU - Suga, Sadao

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

N1 - Funding Information: Grant support: This work was supported in part by a grant-in-aid for the Open Research Center, and the grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan. This study was also conducted with the support of a grant for Research Promotion of Emerging and Re-emerging Infectious Diseases (H18-Shinko-004) from the Ministry of Health, Labour and Welfare of Japan.

PY - 2008/12

Y1 - 2008/12

N2 - Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.

AB - Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.

KW - Genotyping

KW - HHV-6

KW - LAMP

KW - Variant

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Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B

Abstract

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