Loss of function studies in mice and genetic association link receptor protein tyrosine phosphatase α to schizophrenia

Nagahide Takahashi, Karin Sandager Nielsen, Branko Aleksic, Steffen Petersen, Masashi Ikeda, Itaru Kushima, Nathalie Vacaresse, Hiroshi Ujike, Nakao Iwata, Véronique Dubreuil, Naheed Mirza, Takeshi Sakurai, Norio Ozaki, Joseph D. Buxbaum, Jan Sap

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Abstract

Background: Solid evidence links schizophrenia (SZ) susceptibility to neurodevelopmental processes involving tyrosine phosphorylation-mediated signaling. Mouse studies implicate the Ptpra gene, encoding protein tyrosine phosphatase RPTPα, in the control of radial neuronal migration, cortical cytoarchitecture, and oligodendrocyte differentiation. The human gene encoding RPTPα, PTPRA, maps to a chromosomal region (20p13) associated with susceptibility to psychotic illness. Methods: We characterized neurobehavioral parameters, as well as gene expression in the central nervous system, of mice with a null mutation in the Ptpra gene. We searched for genetic association between polymorphisms in PTPRA and schizophrenia risk (two independent cohorts, 1420 cases and 1377 controls), and we monitored PTPRA expression in prefrontal dorsolateral cortex of SZ patients (35 cases, 2 control groups of 35 cases). Results: We found that Ptpra -/- mice reproduce neurobehavioral endophenotypes of human SZ: sensitization to methamphetamine-induced hyperactivity, defective sensorimotor gating, and defective habituation to a startle response. Ptpra loss of function also leads to reduced expression of multiple myelination genes, mimicking the hypomyelination-associated changes in gene expression observed in postmortem patient brains. We further report that a polymorphism at the PTPRA locus is genetically associated with SZ, and that PTPRA mRNA levels are reduced in postmortem dorsolateral prefrontal cortex of subjects with SZ. Conclusions: The implication of this well-studied signaling protein in SZ risk and endophenotype manifestation provides novel entry points into the etiopathology of this disease.

Original languageEnglish
Pages (from-to)626-635
Number of pages10
JournalBiological Psychiatry
Volume70
Issue number7
DOIs
Publication statusPublished - 01-10-2011

All Science Journal Classification (ASJC) codes

  • Biological Psychiatry

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    Takahashi, N., Nielsen, K. S., Aleksic, B., Petersen, S., Ikeda, M., Kushima, I., Vacaresse, N., Ujike, H., Iwata, N., Dubreuil, V., Mirza, N., Sakurai, T., Ozaki, N., Buxbaum, J. D., & Sap, J. (2011). Loss of function studies in mice and genetic association link receptor protein tyrosine phosphatase α to schizophrenia. Biological Psychiatry, 70(7), 626-635. https://doi.org/10.1016/j.biopsych.2011.06.016