Loss of merlin-p85 protein complex in NF2-related tumors

Hideo Takeshima; Toru Nishi; Keizo Yamamoto; Takeshi Kino; Hideo Nakamura; Hideyuki Saya; Masato Kochi; Jun Ichi Kuratsu; Yukitaka Ushio

Loss of merlin-p85 protein complex in NF2-related tumors

Hideo Takeshima, Toru Nishi, Keizo Yamamoto, Takeshi Kino, Hideo Nakamura, Hideyuki Saya, Masato Kochi, Jun Ichi Kuratsu, Yukitaka Ushio

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The NF2 tumor suppressor gene product, designated merlin, belongs to the family of molecules that links membranous protein with the cytoskeleton. We have previously shown that merlin was co-immunoprecipitated with a cellular protein, p85, in cultured cell. To analyze the alteration of merlin and associated proteins in surgical specimens, we developed a new method for biotin-labeling of whole cellular proteins. Screening of tumor tissues using our method showed that none of malignant gliomas and half of the NF2-related tumors had altered p85 and merlin. Our detection method seems useful for the screening of merlin alterations in NF2-related tumors.

Original language	English
Pages (from-to)	1073-1078
Number of pages	6
Journal	International journal of oncology
Volume	12
Issue number	5
Publication status	Published - 05-1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Loss of merlin-p85 protein complex in NF2-related tumors",

abstract = "The NF2 tumor suppressor gene product, designated merlin, belongs to the family of molecules that links membranous protein with the cytoskeleton. We have previously shown that merlin was co-immunoprecipitated with a cellular protein, p85, in cultured cell. To analyze the alteration of merlin and associated proteins in surgical specimens, we developed a new method for biotin-labeling of whole cellular proteins. Screening of tumor tissues using our method showed that none of malignant gliomas and half of the NF2-related tumors had altered p85 and merlin. Our detection method seems useful for the screening of merlin alterations in NF2-related tumors.",

author = "Hideo Takeshima and Toru Nishi and Keizo Yamamoto and Takeshi Kino and Hideo Nakamura and Hideyuki Saya and Masato Kochi and Kuratsu, {Jun Ichi} and Yukitaka Ushio",

year = "1998",

month = may,

language = "English",

volume = "12",

pages = "1073--1078",

journal = "International journal of oncology",

issn = "1019-6439",

publisher = "Spandidos Publications",

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TY - JOUR

T1 - Loss of merlin-p85 protein complex in NF2-related tumors

AU - Takeshima, Hideo

AU - Nishi, Toru

AU - Yamamoto, Keizo

AU - Kino, Takeshi

AU - Nakamura, Hideo

AU - Saya, Hideyuki

AU - Kochi, Masato

AU - Kuratsu, Jun Ichi

AU - Ushio, Yukitaka

PY - 1998/5

Y1 - 1998/5

N2 - The NF2 tumor suppressor gene product, designated merlin, belongs to the family of molecules that links membranous protein with the cytoskeleton. We have previously shown that merlin was co-immunoprecipitated with a cellular protein, p85, in cultured cell. To analyze the alteration of merlin and associated proteins in surgical specimens, we developed a new method for biotin-labeling of whole cellular proteins. Screening of tumor tissues using our method showed that none of malignant gliomas and half of the NF2-related tumors had altered p85 and merlin. Our detection method seems useful for the screening of merlin alterations in NF2-related tumors.

AB - The NF2 tumor suppressor gene product, designated merlin, belongs to the family of molecules that links membranous protein with the cytoskeleton. We have previously shown that merlin was co-immunoprecipitated with a cellular protein, p85, in cultured cell. To analyze the alteration of merlin and associated proteins in surgical specimens, we developed a new method for biotin-labeling of whole cellular proteins. Screening of tumor tissues using our method showed that none of malignant gliomas and half of the NF2-related tumors had altered p85 and merlin. Our detection method seems useful for the screening of merlin alterations in NF2-related tumors.

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M3 - Article

C2 - 9538131

AN - SCOPUS:0031955508

SN - 1019-6439

VL - 12

SP - 1073

EP - 1078

JO - International journal of oncology

JF - International journal of oncology

IS - 5

ER -

Loss of merlin-p85 protein complex in NF2-related tumors

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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